Assays for functional nuclear receptors

• US 5,770,176 A
• Filed: 12/08/1995
• Issued: 06/23/1998
• Est. Priority Date: 12/08/1995
• Status: Expired due to Term

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Chiron Corporation (Bayer AG)
• Inventors: Nargessi, Ruhangiz Dokhi
• Primary Examiner(s): Jones, W. Gary
• Assistant Examiner(s): REES, DIANE
• Application Number:
  US08/569,977
• Time in Patent Office: 928 Days
• Field of Search: 424/1.49, 435/6, 435/91.2, 435/7.1-7.9, 536/23.1, 536/24.3, 536/24.33
• US Class Current: 424/1.49
• CPC Class Codes: G01N 33/566 using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
• CPC Class Codes: G01N 33/574 for cancer

Abstract

Methods and test kits for detecting, or detecting and quantitating functional nuclear receptors in cell or tissue samples are disclosed. Such methods provide highly sensitive assays requiring small sample sizes and short turnaround times. The methods are useful in developing prognoses and/or treatment programs for cancer patients, especially in determining whether therapy to interfere with the receptor'"'"'s activation of gene transcription, such as, endocrine therapy, would be helpful.

Claims

1. A method of detecting the presence or absence of functional nuclear receptors in a cell or tissue sample, or of detecting and quantitating functional nuclear receptors in a cell or tissue sample comprising:

(a) contacting said sample with the following;

(1) ligand to which the nuclear receptor under assay binds;

(2) antibody that is capable of specifically binding to the nuclear receptor under assay when said receptor is bound both to its associated ligand and response element; and

(3) a nucleic acid reagent containing one or more response element segments to which the nuclear receptor under assay binds;

wherein either said antibody or said nucleic acid reagent is attached to a solid phase, and wherein the sample may be contacted with the ligand, the nucleic acid reagent and the antibody components simultaneously, with two of said components simultaneously, or sequentially with said three components in any order; and

(b) correlating the specific binding of said nucleic acid reagent, said ligand and said antibody to a substance in said sample with the presence of functional nuclear receptors in said sample, or correlating the amount of complexes formed by the specific binding of said nucleic acid reagent, said ligand, said antibody and a substance in said sample with the amount of functional nuclear receptors in said sample.

2. The method according to claim 1 wherein the steps are carried out in an automated immunoassay system.

3. The method according to claim 1 wherein said antibody is attached to the solid phase, and wherein said nucleic acid reagent is directly or indirectly linked to a detectable marker;

or wherein said nucleic acid reagent is attached to the solid phase, and wherein said antibody is directly or indirectly linked to a detectable marker.

4. The method according to claim 3 wherein said antibody is attached to the solid phase;

wherein the antibody and ligand are incubated with said sample, and thereafter uncomplexed sample components are removed, before the nucleic acid reagent is added; and

wherein said nucleic acid reagent is directly or indirectly linked to a detectable marker.

5. The method according to claim 3 wherein the nucleic acid reagent is attached to the solid phase and is in excess;

wherein non-specific binding of proteins, other than the nuclear receptor under assay, in the sample to the nucleic acid reagent is prevented by including appropriate non-specific nucleic acids;

wherein the nucleic acid reagent and the ligand are incubated with said sample, and thereafter, sample components uncomplexed to the solid phase are removed, before the antibody is added; and

wherein said antibody is directly or indirectly bound to a detectable marker.

6. The method according to claim 1 wherein the nuclear receptor under assay is a steroid/thyroid hormone receptor selected from the group consisting of steroid hormone receptors, receptors for hormonal forms of vitamin A and D, thyroid hormones, and retinoic acid receptors.

7. The method according to claim 1 wherein the nuclear receptor under assay is selected from the group consisting of steroid hormone receptors.

8. The method according to claim 7 wherein the steroid hormone receptor is selected from the group consisting of estrogen receptor, progesterone receptor, glucocorticoid receptor, mineralocorticoid receptor, androgen receptor, and vitamin D3 receptor.

9. The method according to claim 7 wherein the steroid hormone receptor is selected from the group consisting of estrogen receptor and progesterone receptor.

10. The method according to claim 7 wherein the steroid hormone receptor is an estrogen receptor.

11. The method according to claim 1 wherein said cell or tissue sample is a cell or tissue extract, or a cell lysate.

12. The method according to claim 1 wherein said cell or tissue sample is a cytosol and/or a nuclear extract.

13. The method according to claim 1 wherein said cell or tissue sample is vertebrate.

14. The method according to claim 1 wherein said cell or tissue sample is mammalian.

15. The method according to claim 1 wherein said cell or tissue sample is human.

16. The method according to claim 3 wherein said detectable marker is selected from the group consisting of radionuclides, fluorescers, bioluminescers, chemiluminescers, dyes, enzymes, coenzymes, enzyme substrates, enzyme cofactors, enzyme inhibitors, enzyme subunits, metal ions, and free radicals.

17. The method according to claim 16 wherein the detectable marker is either selected from the group consisting of acridinium esters, acridinium sulfonyl carboxamides, fluorescein, luminol, umbelliferone, isoluminol derivatives, photoproteins, and luciferases, or is produced by an enzymatic reaction upon a substrate.

18. The method according to claim 3 wherein the detectable marker is either an acridinium ester or is produced by an enzymatic reaction with a chemiluminescent substrate and an enzyme selected from the group consisting of alkaline phosphatase, glucose oxidase, glucose 6-phosphate dehydrogenase, α

-, β

-galactosidase, horseradish peroxidase, and xanthine oxidase.

19. The method according to claim 18 wherein the enzyme is alkaline phosphatase, and its substrate is an adamantyl 1,2-dioxetane aryl phosphate with or without an enhancer molecule;

or wherein the enzyme is horseradish peroxidase, and its substrate is 2'"'"', 3'"'"', 6'"'"'-trifluorophenyl 3-methoxy-10-methylacridan-9-carboxylate.

20. The method according to claim 19 wherein the enzyme is alkaline phosphatase, and its substrate is a disodium salt of 4-methoxy-4-(3-phosphatephenyl) spiro 1,2-dioxetane-3,2'"'"'-adamantane! with or without 1-(trioctylphosphonium methyl)-4-(tributylphosphonium methyl) benzene dichloride as an enhancer.

21. The method according to claim 4 wherein said nucleic acid reagent is conjugated to a member of a binding pair, and the detectable marker is conjugated to the other member of said binding pair;

wherein said nucleic acid reagent is incubated with the solid phase complex, and thereafter, uncomplexed reagents are removed, before the conjugated detectable marker is added and then detected, or detected and quantitated.

22. The method according to claim 21 wherein said binding pair comprises biotin and either avidin or streptavidin.

23. The method according to claim 22 wherein the nucleic acid reagent is biotinylated, and the avidin or streptavidin is conjugated to alkaline phosphatase or horseradish peroxidase.

24. The method according to claim 23 wherein the nucleic acid reagent is biotinylated either selectively or randomly at multiple sites.

25. The method according to claim 24 wherein the nucleic acid reagent is biotinylated selectively at the 5'"'"'phosphorylated ends and/or at multiple sites that are not within a response element core sequence.

26. The method according to claim 3 wherein the sample tested is from a biopsied tissue or from a cell suspension from a breast cancer patient, and the nuclear receptor under assay is a steroid hormone receptor.

27. The method according to claim 26 wherein the sample is from a fine needle aspirate or from a stereotactic needle biopsy.

28. The method according to claim 1 wherein the one or more response element segments to which the nuclear receptor under assay binds contain a sequence selected from the group consisting of:

SEQ. ID. NOS.;

1-3 and 5-14.

29. The method according to claim 1 wherein said nucleic acid reagent is double-stranded and contains two to eight response element segments, wherein each of the response element core sequences consists of two inverted repeats, which are the same in each segment, and wherein the central spacer of three base pairs separating the inverted repeats may vary between or among the segments;

and wherein the sequences flanking the core sequences of said response element segments are AT-rich, and may vary in sequence between or among the response element segments.

30. The method according to claim 1 wherein said nucleic acid reagent contains one to fifteen copies of a response element segment.

31. The method according to claim 9 wherein the steroid hormone receptor under assay is an estrogen receptor, and wherein the estrogen response element segment has as its sequence or contains a sequence selected from the group consisting of SEQ. ID. NOS. 3, 7-10 and 12-14.

32. The method according to claim 31 wherein the nucleic acid reagent has SEQ. ID. NO.:

1 as its sequence, or is a monomer, dimer, trimer or tetramer of SEQ. ID. NO. 7.

33. The method according to claim 1 wherein the nucleic acid reagent is DNA that is isolated from naturally occurring sources.

34. The method according to claim 1 wherein the nucleic acid reagent is prepared recombinantly or synthetically.

35. The method according to claim 34 wherein the nuclear receptor under assay is an estrogen receptor;

wherein the nucleic acid reagent contains more than one copy of an estrogen response element segment; and

wherein the nucleic acid reagent is prepared synthetically and selectively biotinylated at the 5'"'"' phosphorylated ends and/or at sites not within any core sequences of said estrogen response element segments.

36. The method according to claim 34 wherein the nucleic acid reagent contains a monomer, dimer, trimer, tetramer, pentamer, septamer or octamer of an estrogen response element segment;

and wherein the nucleic acid reagent is selectively biotinylated at the 5'"'"' phosphorylated ends and/or at multiple sites that are not within the core sequences of the estrogen response elements.

37. The method according to claim 36 wherein the core sequence is contained in a sequence selected from the group consisting of SEQ. ID. NOS.:

7-10 and 12-14.

38. The method according to claim 1 wherein the nucleic acid reagent further comprises one or two oligonucleotide segments that are single-stranded at either or both ends of said nucleic acid reagent, and wherein said oligonucleotide segment(s) is or are complementary to a labeled oligomer probe.

39. The method according to claim 1 wherein said solid phase is particulate.

40. The method according to claim 39 wherein said particulate solid phase comprises magnetic or paramagnetic particles.

41. The method according to claim 1 wherein said solid phase is a solid wall surface of a container or the surface of beads.

42. The method of claim 41 wherein said solid phase is a microtiter plate well.

43. The method according to claim 1 wherein said sample is from a biopsied tissue or from a cell suspension from a patient who has cancer or is suspected of having cancer.

44. The method according to claim 43 wherein the sample is from a fine needle aspirate or from a stereotactic needle biopsy.

45. The method according to claim 43 wherein said cancer is selected from the group consisting of breast, endometrial, uterine, cervical, ovarian, prostrate, vaginal or vulval cancers.

46. The method according to claim 45 wherein the steroid hormone receptor under assay is estrogen receptor, and said cancer is breast cancer.

47. The method according to claim 43 wherein the amount of biopsied tissue necessary for preparing the sample to be assayed is less than 50 mg.

48. The method according to claim 47 wherein the amount of biopsied tissue required is between 10 mg and 25 mg.

49. The method according to claim 1 wherein the antibody is a monoclonal antibody.

50. The method according to claim 1 wherein the antibody is a biologically active antibody fragment.

51. The method according to claim 4 wherein a signal amplification system is used to amplify the signal from the detectable marker.

52. The method according to claim 51 wherein said signal amplification system comprises using a nucleic acid reagent that is biotinylated at multiple sites;

after removing uncomplexed components, adding streptavidin/avidin conjugated to horseradish peroxidase or alkaline phosphatase; and

then after removing uncomplexed components, adding an appropriate chemiluminescent substrate.

53. The method according to claim 51 comprising the use of nucleic acid multimers that each comprise (i) an oligonucleotide unit that is complementary to a segment of the nucleic acid reagent, and (ii) a multiplicity of second oligonucleotide units that are complementary to an oligonucleotide that is labeled directly or indirectly.

54. The method according to claim 1 wherein said antibody is attached to the solid phase, and wherein complexes of said antibody, said ligand, said functional nuclear receptor, and said nucleic acid reagent are detected, or are detected and quantitated by the use of a signal amplification system, comprising:

(a'"'"') contacting said complexes under hybridizing conditions with a nucleic acid multimer, said multimer comprising (i) at least one oligonucleotide unit that is complementary to a segment of the nucleic acid reagent and (ii) a multiplicity of second oligonucleotide units that are complementary to an oligonucleotide that is labeled either directly or indirectly;

(b'"'"') removing unbound multimer;

(c'"'"') contacting under hybridizing conditions the solid phase complex product of step (a'"'"') with the labeled oligonucleotide;

(d'"'"') removing unbound labeled oligonucleotides; and

(e'"'"') detecting signal from any label on the solid phase complex product of step (d'"'"') and correlating that signal with the presence of nuclear receptors under assay that are functional, or detecting and quantitating the amount of signal from label on the solid phase complex product of step (d'"'"') and correlating the amount of signal with the amount of nuclear receptors under assay that are functional.

55. The method according to claim 54 wherein the label is selected from the group consisting of radionuclides, fluorescers, bioluminescers, chemiluminescers, dyes, enzymes, coenzymes, enzyme substrates, enzyme cofactors, enzyme inhibitors, enzyme subunits, metal ions, free radicals and particles.

56. The method according to claim 55 wherein the label is either an acridinium ester or is produced by an enzymatic reaction with an enzyme selected from the group consisting of alkaline phosphatase, glucose oxidase, glucose 6-phosphate dehydrogenase, α

-, β

-galactosidase, horseradish peroxidase, and xanthine oxidase.

57. The method according to claim 56 wherein the enzyme is alkaline phosphatase, and its substrate is an adamantyl 1,2-dioxetane aryl phosphate with or without an enhancer molecule, or is paranitrophenyl phosphate;

or wherein the enzyme is horseradish peroxidase, and its substrate is either tetramethyl benzidine, or 2'"'"', 3'"'"', 6'"'"'-trifluorophenyl 3-methoxy-10-methylacridan-9 carboxylate.

58. The method according to claim 57 wherein the enzyme is alkaline phosphatase, and its substrate is a disodium salt of 4-methoxy-4-(3-phosphatephenyl)spiro 1,2-dioxetane-3,2'"'"'-adamantane! with or without 1-(trioctylphosphonium methyl)-4-(tributylphosphonium methyl) benzene dichloride as an enhancer.

59. The method according to claim 54 wherein the sample is prepared from biopsied tissue from a patient having cancer or suspected of having cancer, wherein the amount of biopsied tissue necessary for preparing said sample is 25 mg or less.

60. A method of developing a prognosis and/or a treatment program for a cancer patient comprising:

(a) performing the method of claim 1 wherein said sample is prepared from biopsied tissue or from a cell suspension from said patient, and determining either that functional nuclear receptors are present or absent in said sample, or that functional nuclear receptors are present and the quantity of said functional nuclear receptors in said sample; and

(b) making a prognosis for said patient and/or a decision on whether endocrine therapy would be useful in a treatment program for said patient based on the results from step (a).

61. The method according to claim 60 wherein said cancer patient has cancer of the breast, endometrium, uterus, cervix, ovaries, prostate, vagina or vulva.

62. The method according to claim 60 wherein the nuclear receptor under assay is human estrogen receptor;

wherein the patient is a breast cancer patient; and

wherein the presence of functional estrogen receptors detected, or detected and quantitated in said sample, indicates a positive prognosis, and that endocrine therapy would be helpful in a treatment program for said patient.

63. The method according to claim 62 wherein said endocrine therapy is selected from the group consisting of administration of anti-estrogen compounds, performance of an ovariectomy, performance of a hypophysectomy, and the administration of androgen.

64. A test kit for performing the method of claim 1 comprising:

(a) an antibody that is capable of specifically binding to the nuclear receptor under assay when said receptor is bound both to its associated ligand and response element;

(b) ligand to the nuclear receptor under assay; and

(c) a nucleic acid reagent containing one or more response element segments to which the nuclear receptor under assay binds;

wherein either said antibody or said nucleic acid reagent is attached to a solid phase.