Matrix-bearing targets for maldi mass spectrometry and methods of production thereof

• US 5,770,272 A
• Filed: 04/28/1995
• Issued: 06/23/1998
• Est. Priority Date: 04/28/1995
• Status: Expired due to Fees

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Kochling, Heinrich, Biemann, Klaus
• Primary Examiner(s): Beck, Shrive
• Assistant Examiner(s): CHEN, BRET P
• Application Number:
  US08/431,064
• Time in Patent Office: 1,152 Days
• Field of Search: 427/421, 427/424, 427/422, 250/288
• US Class Current: 427/162
• CPC Class Codes: B05B 12/18 using fluids, e.g. gas streams
• CPC Class Codes: H01J 49/0418 for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates

Abstract

The present invention provides new methods for producing substantially continuous, homogeneous layers of MALDI matrix materials deposited on MALDI targets and substantially free of voids and large crystals. The methods involve the deposition of MALDI matrix materials in a nebulized spray which is enveloped in a sheath of non-reactive gas which confines and entrains the spray and aids in the evaporation of the solvent such that substantial, if not complete, solvent evaporation occurs before the matrix material is deposited on the target surface. The invention further provides such matrix layers and pre-formed matrix-bearing targets for use in MALDI mass spectrometry.

Claims

1. A method of forming a continuous matrix-bearing target having a matrix aver with an average thickness in excess of 0.7 μ

m and being substantially free of matrix crystals having any dimension in excess of 10 μ

m for matrix-assisted laser desorption/ionization mass spectrometry comprising;

directing at a deposition surface a spray of a solution of a matrix-assisted laser desorption/ionization matrix material dissolved in a solvent;

simultaneously directing at said surface a stream of non-reactive gas forming a substantially coaxial sheath enveloping said spray; and

causing said surface and said spray to move relative to one another forming a continuous matrix layer of said matrix material having an average thickness in excess of 0.7 μ

m and being substantially free of matrix crystals having any dimension in excess of 10 μ

m the matrix layer being deposited on said surface.

2. A method as in claim 1 wherein said matrix material is selected from the group consisting of sinapinic acid, α

-cyano-4-hydroxycinnamic acid, 2,5-dihydroxybenzoic acid, 3-hydroxypicolinic acid, 5-(trifluoro-methyl)uracil, caffeic acid, succinic acid, anthranilic acid, 3-aminopyrazine-2-carboxylic acid, ferulic acid, 7-amino-4-methyl-coumarin, 2,4,6-trihydroxy acetophenone, and 2-(4-hydroxyphenylazo)-benzoic acid.

3. A method as in claim 1 wherein said non-reactive gas is selected from the group consisting of N₂, the noble gases, and dried air.

4. A method as in claim 1 wherein said non-reactive gas is heated relative to said solution.

5. A method as in claim 1 further comprising the steps of allowing said matrix material to crystallize on said surface and contacting said matrix material with a non-abrasive material to remove a layer of loose microcrystals.