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Nucleic acid capture moieties

  • US 5,770,365 A
  • Filed: 08/25/1995
  • Issued: 06/23/1998
  • Est. Priority Date: 08/25/1995
  • Status: Expired due to Term
First Claim
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1. A method of promoting the hybridization of a target single-stranded nucleic acid and a nucleic acid capture moiety comprising:

  • a) providing a unimolecular nucleic acid capture moiety having a double-stranded region with a terminal base and an adjacent single-stranded region, the terminal base of the double-stranded region being all or part of a duplex-binding-ligand binding site, and said single-stranded region being capable of hybridizing to said target nucleic acid, wherein said unimolecular nucleic acid capture moiety is bound to a solid support;

    b) forming a reaction mixture comprising said target single-stranded nucleic acid, said nucleic acid capture moiety, and a duplex-binding ligand, under conditions such that the target single-stranded nucleic acid and the nucleic acid capture moiety can hybridize; and

    c) allowing said target single-stranded nucleic acid to hybridize to said nucleic acid capture moiety such that said duplex-binding ligand binds to the intermolecular duplex thereby formed,such that the hybridization of the target single-stranded nucleic acid and the nucleic acid capture moiety is promoted.

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