×

Tag reagent and assay method

  • US 5,770,367 A
  • Filed: 02/05/1996
  • Issued: 06/23/1998
  • Est. Priority Date: 07/30/1993
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method of sequencing a target nucleic acid, which method comprises the steps of:

  • a) providing an oligonucleotide immobilized on a support,b) hybridizing the target nucleic acid with the immobilized oligonucleotide,c) incubating the hybrid from b) with a library comprising a solution of reagents, each of said reagents comprising1) an analyte moiety comprising at least two analyte residues, and linked to2) a tag moiety comprising one or more reporter groups suitable for detection by mass spectrometry, excluding oligonucleotides.wherein a reporter group designates an analyte residue, and the reporter group at each position of the tag moiety is chosen to designate an analyte residue at a defined position of the analyte moietyand wherein the library consists of 4n reagents, each of said reagents comprising a different analyte moiety which is a different oligonucleotide chain of n nucleotides,so that an oligonucleotide chain of a first reagent of the library becomes hybridized to the target nucleic acid adjacent the immobilized oligonucleotide,d) ligating the adjacent oligonucleotides, thus forming a ligated first reagent,e) removing other non-ligated reagents, andf) recovering and analyzing the tag moiety of the ligated first reagent as an indication of the sequence of a first part of the target nucleic acid wherein the said target nucleic acid and ligated first reagent remain hybridized.

View all claims
  • 3 Assignments
Timeline View
Assignment View
    ×
    ×