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Method of separation employing magnetic particles and second medium

  • US 5,770,388 A
  • Filed: 12/13/1993
  • Issued: 06/23/1998
  • Est. Priority Date: 12/22/1989
  • Status: Expired due to Fees
First Claim
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1. A method for the separation of a particulate biologic material (PBM) from a mixture containing said PBM and other components, which method comprises:

  • combining in a first liquid medium said PBM and said other components, and magnetic particles having immobilized thereon a member of a specific binding pair (sbp) that specifically binds to said PBM to form a complex of said PBM and said magnetic particles (PBMMP),layering said first liquid medium with a second liquid medium that is of different density and/or viscosity than said first liquid medium such that said first medium is layered above said second medium,subjecting said first liquid medium and said second liquid medium to a magnetic field gradient whereby said PBMMP migrates into said second liquid medium and is washed thereby, andseparating said PBMMP from said second medium.

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