Apparatus and method for determining the quanity of an analyte in a biological sample by means of transmission photometry

• US 5,770,389 A
• Filed: 02/14/1996
• Issued: 06/23/1998
• Est. Priority Date: 09/27/1993
• Status: Expired due to Term

• Current Assignee: Abbott Laboratories
• Original Assignee: Abbott Laboratories
• Inventors: Stimpson, Donald Irvine, Ching, Shan-Fun, Hoijer, Joanell Veronica, Gordon, Julian
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US08/601,514
• Time in Patent Office: 860 Days
• Field of Search: 307/311, 377/12, 377/20, 377/53, 377/58, 377/95, 356/432-434, 250/214 RC, 250/214 LS, 250/214 SW, 250/214 A, 250/214 PR, 250/214.1, 422/56, 422/57, 422/82.05, 422/82.08, 422/82.09, 422/82.01, 422/82.02, 435/7.9, 435/7.92, 435/7.93, 435/7.94, 435/11, 435/14, 435/287.1, 435/287.2, 435/287.7, 435/287.9, 435/805, 435/808, 435/810, 435/970, 436/46, 436/50, 436/66, 436/164, 436/169, 436/172, 436/514, 436/518-520, 436/524, 436/525, 436/527, 436/528, 436/531, 436/533, 436/534, 436/805, 436/807, 436/808, 436/810
• US Class Current: 435/7.92
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

A device and method for quantitative determination of an analyte in a biological sample utilizes a non-transparent support medium for retaining a chromatogenic reaction product with the medium being exposed to a source of light for transmitting therethrough a scattered, uniform response light signal which is collected at a photosensitive device whereby the amount of the analyte is correlated to the intensity of the response light signal. The response light signal may be converted to a time-duration signal proportional to light intensity to facilitate the quantitative determination.

Claims

1. An apparatus for making a quantitative determination of the amount of an analyte in a biological sample comprising:

a. a support medium for receiving a biological sample and retaining a labeled reaction product thereon, the medium including a read window capable of receiving a source light signal and transmitting therethrough a response light signal that has been altered from the source light signal by the presence of a labeled reaction product, wherein said medium is highly light-scattering, the amount of said alteration of said response light signal by said labeled reaction product being an indication of the amount of said analyte in said biological sample;

b. a source of light positioned to transmit a source light signal to the read window of the medium and to the labeled reaction product at the read window, whereby the response light signal is produced;

c. a collector positioned to receive the response light signal transmitted from the read window of the medium, said collector capable of developing a variable output signal in direct correspondence to the intensity of the response light signal, said collector being a photosensitive element; and

d. means for measuring the variable output signal and correlating the measured signal to the amount of analyte in the sample, said means for measuring the variable output signal comprising;

(1) a capacitor network connected in series with the photosensitive element, said capacitor network comprising a capacitor, said capacitor network having a discharge cycle over a time cycled duration;

(2) a power source coupled to the capacitor network and the photosensitive element and capable of charging the capacitor network; and

(3) means for measuring the duration of the discharge cycle of the capacitor network through the resistance developed by the photosensitive element in response to the intensity of the response light signal, wherein the light intensity of the response light signal is related to the duration of the discharge cycle.

2. The apparatus of claim 1, further including a switching element for selectively activating and deactivating the capacitor network.

3. The apparatus of claim 2, wherein the switching element is a transistor switch capable of allowing the capacitor network to discharge when the transistor is in the ON condition.

4. The apparatus of claim 1, wherein the photosensitive element is a phototransistor.

5. The apparatus of claim 1, wherein the switching element is in parallel with the capacitor network.

6. The apparatus of claim 1, wherein the means for measuring the duration of the discharge cycle comprises a comparator having a reference level signal against which the response light signal is compared to a reference point to determine the intensity of the response light signal.

7. The apparatus of claim 1, wherein the light intensity of the response light signal is directly proportional to the duration of the charge cycle of the capacitor network.

8. The apparatus of claim 1, wherein the source of light is positioned on one side of the support medium and the collector is positioned on the other side of the support medium for receiving the response light signal transmitted therethrough, said source of light and said collector positioned in line with said read window.

9. The apparatus of claim 1, wherein the support medium is a nitrocellulose membrane.

10. The apparatus of claim 1, wherein the label of the labeled reaction product is a chromogen.

11. The apparatus of claim 10, wherein the chromogenic reaction product is the product formed as the result of a chemical or immunological reaction between the analyte or an analog of the analyte and a reagent used in the assay.

12. The apparatus of claim 11, wherein the chromogenic reaction product is an immunologically bound reaction product.

13. The apparatus of claim 1, wherein the analyte is hemoglobin.

14. The apparatus of claim 1, wherein the analyte is cholesterol.

15. The apparatus of claim 1, wherein the analyte is glucose.

16. A method of determination the amount of an analyte present in a liquid biological sample comprising:

a. providing a highly light-scattering support medium for receiving a liquid, analyte-containing biological sample and for retaining thereon a labeled reaction product, said support medium having a read window, said read window being positioned between a source light emitter and a collector, said collector being a photosensitive element;

b. combining said sample with one or more reagents to produce a labeled reaction product, wherein at least one of said one or more reagents is a substance capable of reacting with another assay reagent or the analyte to produce a labeled reaction product or signal that indicates the presence of the analyte and that is detectable by visual or instrumental means;

c. retaining said labeled reaction product on said support medium;

d. exposing said labeled reaction product and said support medium to said source of light whereby there is transmitted through said support medium a response light signal altered from the source of light by the presence of the labeled reaction product, the amount of said alteration of said response light signal by said labeled reaction product being an indication of the amount of analyte in said biological sample;

e. measuring the intensity of the response light signal by generating a variable resistance signal that corresponds to the intensity of the response light signal, said variable resistance signal being measured by a measuring means comprising;

(1) a capacitor network connected in series with the photosensitive element, said capacitor network comprising a capacitor, said capacitor network having a discharge cycle over a time cycled duration;

(2) a power source coupled to the capacitor network and the photosensitive element and capable of charging the capacitor network; and

(3) means for measuring the duration of the discharge cycle of the capacitor network through the resistance developed by the photosensitive element in response to the intensity of the response light signal, wherein the light intensity of the response light signal is related to the duration of the discharge cycle; and

f. correlating the intensity of said response light signal to the amount of analyte in the sample.

17. The method of claim 16, wherein the support medium is formed from a chromatographic medium.

18. The method of claim 16, wherein the generation of said variable resistance signal comprises:

a. charging a capacitor network connected in series with the photosensitive element; and

b. measuring the duration of the discharge cycle of the capacitor network through the resistance developed by the photosensitive element in response to the intensity of the response light signal.

19. The method of claim 18, wherein the photosensitive element is a phototransistor.

20. The method of claim 18, wherein the measuring step includes comparing the time duration of the discharge cycle with a reference time corresponding to a predetermined amount of chromogenic reaction product.

21. The method of claim 18, wherein the light intensity of the response light signal is directly proportional to the duration of the charge cycle of the capacitor network.

22. The method of claim 16, wherein the support medium is a nitrocellulose membrane.

23. The method of claim 16, wherein the labeled reaction product is the product formed as the result of a chemical or immunological reaction between the analyte or an analog of the analyte and a reagent used in the assay.

24. The method of claim 16, wherein the analye is hemoglobin.

25. The method of claim 16, wherein the analye is cholesterol.

26. The method of claim 16, wherein the analyte is glucose.

27. The method of claim 16, wherein at least one of said reagents for the labeled reaction product is a member of a specific binding pair.

28. The method of claim 16, wherein the labeled reaction product is an immunologically bound reaction product.

29. The method of claim 16, wherein the combining of the sample and one or more of said reagents is performed within said support medium.

30. The method of claim 16, wherein said sample and at least one of said one or more reagents are combined external to said support medium.

31. The method of claim 16, wherein said support medium contains a predetermined amount of at least one of said one or more reagents.

32. The method of claim 16, wherein the label of said labeled reaction product is a chromogen.

33. The method of claim 16, wherein the label of said labeled reaction product is fluorogenic.

34. The method of claim 33, wherein the fluorogenic label is an enzyme.

35. The method of claim 16, wherein the label of said labeled reaction product is a particle.

36. The method of claim 35, wherein the particle is colored.

37. The method of claim 35, wherein the particle is a colloidal metal.

38. The method of claim 35, wherein the particle is non-metallic.