Rapid oneside single targeting (ROST) immunoassay method
First Claim
Patent Images
1. A method for the determination of the amount of an antigen, comprising:
- a) incubating said antigen and a first antibody in the presence of an immobilized second antibody, wherein said first antibody is specifically reactive with said antigen, and said second antibody is immobilized on a matrix and binds said first antibody if said first antibody is not bound to said antigen, but does not bind said first antibody if said first antibody is bound to said antigen;
b) separating first antibody in a supernatant from first antibody bound to said second antibody immobilized on said matrix; and
c) determining the amount of said first antibody in said supernatant or the amount of said first antibody bound to said second antibody immobilized on said matrix to give an indication of the amount of said antigen.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to an immunoassay and a method for the determination of antigens, in which an antigen is bound to a specific antibody, the conjugate formed is reacted with an immobilized capture antibody which recognizes and binds the non-bound antibody and the quantity of free or bound antibody is determined in the supernatant or on the matrix.
76 Citations
5 Claims
-
1. A method for the determination of the amount of an antigen, comprising:
-
a) incubating said antigen and a first antibody in the presence of an immobilized second antibody, wherein said first antibody is specifically reactive with said antigen, and said second antibody is immobilized on a matrix and binds said first antibody if said first antibody is not bound to said antigen, but does not bind said first antibody if said first antibody is bound to said antigen; b) separating first antibody in a supernatant from first antibody bound to said second antibody immobilized on said matrix; and c) determining the amount of said first antibody in said supernatant or the amount of said first antibody bound to said second antibody immobilized on said matrix to give an indication of the amount of said antigen. - View Dependent Claims (2, 3, 4, 5)
-
Specification
-
- Resources
Litigation Campaign Assessment
Thank you for your request. You will receive a custom alert email when the Litigation Campaign Assessment is available.
×
-
Current AssigneeBayer AG
-
Original AssigneeBayer AG
-
InventorsStocker, Ronald Helmut, Doth, Margit
-
Primary Examiner(s)Scheiner, Toni R.
-
Application NumberUS08/737,901Time in Patent Office580 DaysField of Search435/7.1, 435/7.93, 435/7.92, 435/5, 436/518US Class Current436/518CPC Class CodesG01N 33/538 by sorbent column, particle...G01N 33/541 Double or second antibody ,...G01N 33/54306 Solid-phase reaction mechan...
