Method of manufacturing a matrix for the detection of mismatches
First Claim
1. A method to prepare a patterned matrix for immobilizing oligonucleotides at distinct intervals on said matrix, comprising:
- (a) precoating a solid support at distinct intervals with a sublayer of a substance to form a precoated matrix with a prescribed topology, wherein said solid support is virtually transparent to laser radiation and wherein said sublayer substance absorbs laser radiation;
(b) applying a continuous gel coat onto said precoated matrix wherein said continuous gel coat is also virtually transparent to laser radiation to form a gel coated matrix;
(c) irradiating said gel coated matrix with a laser beam so that upon irradiation those parts of the continuous gel coat which are immediately above said sublayer substance are evaporated together with said substance, wherein said evaporation forms a multiplicity of gel cells in the gel coated matrix which are spaced from one another by intervals forming a patterned matrix; and
(d) immobilizing oligonucleotides from a chosen set of oligonucleotides into the gel cells wherein one oligonucleotide is immobilized per gel cell.
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Abstract
This method for preparing micromatrices consists in applying a specially-patterned intermediate layer of laser-absorbing substance on a solid support. The configuration of the sublayer fully corresponds to the topology of the manufactured matrix. The intermediate layer is further covered by a continuous layer of gel , the gel and the material of the support being transparent towards laser radiation. The gel layer is irradiated by a laser beam for a time needed to evaporate simultaneously the gel in the places immediately above the laser-absorbing sublayer and the sublayer itself. Oligonucleotides from a chosen set are then attached to the formed gel `cells`, one oligonucleotide to each cell.
This method is intended for use in biotechnology, specifically for deciphering the nucleotide sequence of DNA.
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Citations
4 Claims
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1. A method to prepare a patterned matrix for immobilizing oligonucleotides at distinct intervals on said matrix, comprising:
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(a) precoating a solid support at distinct intervals with a sublayer of a substance to form a precoated matrix with a prescribed topology, wherein said solid support is virtually transparent to laser radiation and wherein said sublayer substance absorbs laser radiation; (b) applying a continuous gel coat onto said precoated matrix wherein said continuous gel coat is also virtually transparent to laser radiation to form a gel coated matrix; (c) irradiating said gel coated matrix with a laser beam so that upon irradiation those parts of the continuous gel coat which are immediately above said sublayer substance are evaporated together with said substance, wherein said evaporation forms a multiplicity of gel cells in the gel coated matrix which are spaced from one another by intervals forming a patterned matrix; and (d) immobilizing oligonucleotides from a chosen set of oligonucleotides into the gel cells wherein one oligonucleotide is immobilized per gel cell. - View Dependent Claims (2, 3, 4)
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Specification