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Method of preparing gas-filled microspheres using a lyophilized lipids

  • US 5,776,429 A
  • Filed: 04/30/1996
  • Issued: 07/07/1998
  • Est. Priority Date: 12/22/1989
  • Status: Expired due to Term
First Claim
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1. A method of preparing a gas-filled microsphere comprising:

  • a. obtaining a lyophilized lipid composition comprising the lipids dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine-polyethylene glycol, and dipalmitoylphosphatidic acid, in a ratio of about 70 to about 90 mole percent, about 5 to about 15 mole percent, and about 5 to about 15 mole percent, respectively, wherein the combined concentration of lipids is about 20 mg/ml to about 50 mg/ml of aqueous solution prior to lyophilizing;

    b. dispersing said lyophilized composition in an aqueous-based pharmaceutically acceptable carrier to a concentration of about 0.1 mg/ml to about 5 mg/ml to form an aqueous microsphere-forming solution;

    c. introducing a fluorine-containing gas into said aqueous microsphere-forming solution; and

    d. shaking said aqueous microsphere-forming solution to form a microsphere filled with fluorine-containing gas.

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