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Methods for detection by in situ hybridization of multiple chromosomes or regions thereof

  • US 5,776,688 A
  • Filed: 01/10/1997
  • Issued: 07/07/1998
  • Est. Priority Date: 09/20/1990
  • Status: Expired due to Term
First Claim
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1. A method for the detection by in situ hybridization of a plurality of target chromosomes or a plurality of target regions of chromosomes comprising:

  • (a) contacting under hybridizing conditions a plurality of target chromosomes or a plurality of target regions of chromosomes, which are contained within a cytological or histological preparation or specimen, with a multiple probe composition comprising a plurality of direct label DNA probe compositions, wherein;

    (i) each of the direct label DNA probe compositions is detectable by use of a different fluorescent label, each label being detectable in the presence of the other fluorescent label or labels, and(ii) each of the direct label DNA probe compositions comprises direct label DNA segments which;

    (1) are complementary to different target chromosomes or target regions of a chromosome to be detected,(2) have multiple fluorescent labels covalently attached thereto, and(3) have 0.3 to 6 mole percent of total nucleotides in each direct label DNA segment fluorescently labeled, and(b) detecting the presence or absence of hybridization of each of the direct label DNA probe compositions to the chromosomes or regions of a chromosome by fluorescent techniques.

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