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Materials and methods for detecting oxalate

  • US 5,776,701 A
  • Filed: 05/31/1996
  • Issued: 07/07/1998
  • Est. Priority Date: 05/31/1996
  • Status: Expired due to Fees
First Claim
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1. A process for preparing an oxalate oxidase composition comprising the steps of preparing an extract from plant tissue, wherein phenylmethylsulfonyl flouride is included during preparation of said extract, precipitating proteins in said extract by bringing said extract to at least about 30% saturation with ammonium sulfate, removing said precipitated proteins and bringing said extract to at least about 60% saturation with ammonium sulfate to precipitate said oxalate oxidase present in said extract, and recovering said oxalate oxidase.

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