Modification of vegetable oils using desaturase

• US 5,777,201 A
• Filed: 10/31/1996
• Issued: 07/07/1998
• Est. Priority Date: 03/13/1992
• Status: Expired due to Term

• Current Assignee: Agrigenetics Incorporated (Corteva, Inc.)
• Original Assignee: Agrigenetics Incorporated (Corteva, Inc.)
• Inventors: Poutre, Candace Gloria, Mchra-Palta, Asha
• Primary Examiner(s): RORIES, CHARLES
• Application Number:
  US08/742,273
• Time in Patent Office: 614 Days
• Field of Search: 800/205, 800/250, 800/255, 800/DIG. 17, 800/DIG. 56, 800/DIG. 9, 800/DIG. 52, 800/DIG. 69, 435/172.3, 435/375, 435/69.1
• US Class Current: 800/281
• CPC Class Codes: C12N 15/82 for plant cells , e.g. plant artificial chromosomes (PACs)
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 9/0004 Oxidoreductases (1.)
• CPC Class Codes: C12N 9/0083 Miscellaneous (1.14.99)
• CPC Class Codes: C12Y 114/19002 Acyl-[acyl-carrier-protein] desaturase (1.14.19.2)

Abstract

Plant seed having yeast delta-9 desaturase gene therein, preferably in association with a suitable promoter and termination sequence. A method for modifying the fatty acid content of seed oil, by transforming seed with yeast delta-9 desaturase.

Claims

1. A plant seed comprising DNA encoding yeast delta-9 desaturase and means for expressing said DNA in said plant seed.

2. The plant seed as defined by claim 1, wherein said means for expressing comprises a promoter effective to cause expression of said DNA in said plant seed.

3. The plant seed as defined by claim 2, wherein said promoter is a seed-specific promoter.

4. The plant seed as defined by claim 1, further comprising a termination sequence for said DNA.

5. The plant seed as defined by claim 4, wherein said termination sequence is selected from the group consisting of a yeast delta-9 desaturase termination sequence (SEO ID NO:

1), a phaseolin 3'"'"' termination sequence, and an ORF 25 3'"'"' termination sequence.

6. The plant seed as defined by claim 1, wherein said plant seed is a seed of a monocot plant genus.

7. The plant seed as defined by claim 6, wherein said monocot plant genus is selected from the group consisting of Zea and Sorghum.

8. The plant seed as defined by claim 1, wherein said plant seed is a seed of a dicot plant genus.

9. The plant seed as defined by claim 8, wherein said dicot plant genus is selected from the group consisting of Brassica, Helianthus, Carthamus, Sesamum, Glycine, Arachis, Gossypium, Lesquerella, and Vernonia.

10. The plant seed as defined by claim 9, further wherein said plant seed is a seed selected from the group consisting of Brassica rapa and Brassica napus.

25. A plant obtained from the plant seed as defined by claim 1, wherein the seeds of said plant comprise a DNA encoding yeast delta-9 desaturase and means for expressing said DNA in said plant seed.

11. A method for providing a modified fatty acid content of the seed oil of a plant seed, said method comprising the step of providing a transgenic plant seed comprising DNA encoding yeast delta-9 desaturase, wherein said DNA is expressed, whereby the fatty acid content is modified relative to the seed oil of non-transgenic plant seed.

12. The method as defined by claim 11, wherein said modification comprises an increase in the percent content of monounsaturated fatty acid in the seed oil of said plant seed.

13. The method as defined by claim 12, wherein said monounsaturated fatty acid has a carbon chain length of from 16 to 24 carbon atoms.

14. The method as defined by claim 13, wherein said monounsaturated fatty acid is selected from the group consisting of cis-9-hexadecanoic acid (palmitoleic acid), cis-9-octadecanoic acid (oleic acid), cis-11-octadecenoic acid (cis-vaccenic acid), cis-11-eicosenoic acid, cis-13-eicosenoic acid, cis-13-docosenoic acid, cis-15-docosenoic acid, cis-15-tetracosenoic acid, cis-17-tetracosenoic acid, and combinations thereof.

15. The method as defined by claim 11, wherein said modification comprises a reduction in the percent content of saturated fatty acid in the seed oil of said plant seed.

16. The method as defined by claim 15, wherein said saturated fatty acid is selected from the group consisting of myristic acid, palmitic acid, stearic acid, eicosanoic acid, docosanoic acid, tetracosanoic acid, and combinations thereof.

17. The method as defined by claim 15, wherein said plant seed is a seed of a monocot plant genus.

18. The method as defined by claim 17, wherein said monocot plant genus is selected from the group consisting of Zea and Sorghum.

19. The method as defined by claim 11, wherein said plant seed is a seed of a dicot plant genus.

20. The method seed as defined by claim 19, wherein said dicot plant genus is selected from the group consisting of Brassica, Helianthus, Carthamus, Sesamum, Glycine, Arachis, Gossypium, Lesquerella, and Vernonia.

21. The method as defined by claim 20, further wherein said plant seed is a seed selected from the group consisting of Brassica rapa and Brassica napus.

22. The method as defined by claim 11, wherein said step of providing comprises transforming a plant cell to contain exogenous DNA encoding yeast delta-9 desaturase, culturing said cell under conditions whereby a plant is regenerated therefrom, and maintaining said plant under conditions whereby said plant produces transgenic seed.

23. The method as defined by claim 22, wherein said step of transforming is carried out using transformation mediation selected from the group consisting of Agrobacterium, electroporation, polyethylene glycol (PEG), silicon carbide fiber, particle gun, and direct injection.

24. The method as defined by claim 23, further comprising constructing a vector containing DNA encoding yeast delta-9 desaturase and a promoter, placing said vector into a selected strain of Agrobacterium, and treating selected plant cells with said Agrobacterium under conditions sufficient to result in transfer of at least some of said vectors from said Agrobacterium to said plant cells, whereby said DNA is expressed in said plant cells.