Laser doppler microscopy methods and instruments
First Claim
Patent Images
1. An instrument for use in the determination of blood cell velocity in a capillary in a tissue by a laser Doppler technique, said instrument comprising:
- means for generating a laser beam;
means for directing the laser beam on to a surface of the tissue so as to impinge on a capillary within which blood cells are traveling with a component of velocity substantially perpendicular to the surface of the tissue;
a camera having an image plane for observing impingement of the laser beam on the surface;
means for measuring the velocity by detecting directly back-scattered laser radiation; and
a lens system which collects the back-scattered laser beam and focuses the Doppler-shifted back-scattered radiation on to a photodetector via at least one beam splitter, said lens system also serving to effect focussing of radiation back-scattered by surrounding tissue and not subjected to Doppler-shifting.
0 Assignments
0 Petitions
Accused Products
Abstract
The velocity of blood cells in skin or other tissue capillaries is determined by a laser Doppler technique. A laser beam is focussed on to a capillary by means of a lens, mirror and beam splitter system and measurement of the velocity of the blood cells in a direction substantially perpendicular to the surface of the tissue is effected by detecting directly back-scattered radiation.
-
Citations
5 Claims
-
1. An instrument for use in the determination of blood cell velocity in a capillary in a tissue by a laser Doppler technique, said instrument comprising:
-
means for generating a laser beam; means for directing the laser beam on to a surface of the tissue so as to impinge on a capillary within which blood cells are traveling with a component of velocity substantially perpendicular to the surface of the tissue; a camera having an image plane for observing impingement of the laser beam on the surface; means for measuring the velocity by detecting directly back-scattered laser radiation; and a lens system which collects the back-scattered laser beam and focuses the Doppler-shifted back-scattered radiation on to a photodetector via at least one beam splitter, said lens system also serving to effect focussing of radiation back-scattered by surrounding tissue and not subjected to Doppler-shifting. - View Dependent Claims (2, 3, 4, 5)
-
Specification