Measuring assembly for luminescence analysis

• US 5,779,978 A
• Filed: 02/07/1997
• Issued: 07/14/1998
• Est. Priority Date: 02/29/1996
• Status: Expired due to Term

• Current Assignee: Avl Medical Instruments AG (Roche Holding AG)
• Original Assignee: Avl Medical Instruments AG (Roche Holding AG)
• Inventors: Hartmann, Paul, Ziegler, Werner, Harer, Johann, Karpf, Hellfried
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US08/797,249
• Time in Patent Office: 522 Days
• Field of Search: 422/82.05, 422/82.06, 422/82.07, 422/82.08, 422/82.11, 422/116, 436/172, 356/244, 356/246
• US Class Current: 422/82.05
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence

Abstract

A measuring assembly comprises a supporting element transparent to excitation and measurement radiation, which is provided with a number of preferably different luminescence-optical sensor elements on a first boundary face. Via a second boundary face excitation radiation can be picked up, and a third boundary face is used to supply measurement radiation of the sensor elements to a detector of an evaluation unit. The direction of the excitation radiation is essentially normal to that of detection. The preferably different sensor elements are connected by a common sample channel, and optical and/or temporal separation of the measurement radiation of the individual sensor elements is provided.

Claims

1. A measuring assembly comprising at least one radiation source, at least one detector and a supporting element being transparent to excitation and measurement radiation and having first, second and third boundary faces, said supporting element being provided with several luminescence-optical sensor elements on said first boundary face, and picks up excitation radiation from said radiation source via said second boundary face, and supplies measurement radiation of said sensor elements to said detector of an evaluation unit via said third boundary face, the direction of said excitation radiation being essentially normal to the direction of detection, and the refractive index n₁ of said supporting element being greater than the refractive index of the environment, wherein said several sensor elements are connected by a common sample channel, and wherein means are provided for optical or temporal separation of light paths of said measurement radiation emitted by said individual sensor elements.

2. A measuring assembly according to claim 1, wherein said supporting element exhibits, along said sample channel and normal thereto, constrictions, recesses, layers or regions being impervious to said measurement radiation, for the purpose of optically separating said light paths of said measurement radiation emitted by said individual sensor elements and wherein each of said sensor elements is assigned a detector of said evaluation unit.

3. A measuring assembly according to claim 2, wherein said supporting element essentially features a comb structure carrying said individual sensor elements along its base said comb structure showing essentially prism-shaped projections in the area of each of said sensor elements, wherein the front faces of said projections are furnished with said detectors.

4. A measuring assembly according to claim 1, wherein said supporting element comprises optoelectronic switching elements situated in said light path of said measurement radiation of each of said sensor elements for opening only one light path at a time between one of said sensor elements and said at least one detector of said evaluation unit.

5. A measuring assembly according to claim 1, wherein each of said sensor elements is provided with a separate radiation source, and all of said sensor elements are assigned one common detector of said evaluation unit.

6. A measuring assembly according to claim 5, wherein said separate radiation sources are connected to an electronic control unit permitting the emission of excitation radiation sequenced in time, such that separation of said light paths of said measurement radiation of each of said sensor elements is achieved by time-shifted excitation of said sensor elements.

7. A measuring assembly according to claim 5, wherein said separate radiation sources are connected to an electronic device for periodic modulation of said excitation radiation, and wherein said detector is connected with a device for periodically modulated detection of said measurement radiation, the light paths of said measurement radiation of each of said sensor elements being separated by measuring the phase angle or the demodulation between said excitation radiation and said measurement radiation.

8. A measuring assembly according to claim 5, wherein said separate radiation sources are furnished with a device for pulsed excitation of said sensor elements, and wherein said detector is connected with a device for time-sequenced detection of said measurement radiation.

9. A measuring assembly according to claim 5, wherein said supporting element features delimited regions in the area of each of said sensor elements, said delimited regions having a refractive index n₂ being greater than the refractive index n₁ of said supporting element outside of said delimited regions, wherein said excitation radiation is fed in in said delimited regions with refractive index n₂ and said measurement radiation is carried off in the remaining regions of said supporting element having a refractive index n₁.

10. A measuring assembly according to claim 1, wherein all of said sensor elements are in optical connection with a common, pulsed radiation source and a common detector, said detector being connected to a device for time-sequenced detection of said measurement radiation in order to separate the light paths of said measurement radiation of each of said sensor elements.

11. A measuring assembly according to claim 1, wherein all of said sensor elements are in optical connection with a common detector, which is provided with a device for simultaneous detection of decay time profiles of all of said sensor elements, and wherein said evaluation unit includes a subunit for mathematical splitting of the decay time functions of said individual sensor elements.

12. A measuring assembly according to claim 1, wherein said sensor elements are combined into pairs, each of said pairs being assigned a separate radiation source, and wherein said evaluation unit is connected to first and second detectors, each first sensor element of a pair of sensor elements being assigned said first detector via a first light path in said supporting element, and each second sensor element of a pair of sensor elements being assigned said second detector via a second light path in said supporting element, and wherein the separation of said light paths of different pairs of sensor elements being effected by time-sequenced excitation of said individual pairs.

13. A measuring assembly according to claim 1, wherein said supporting element is suited for light guidance between said sensor elements and said at least one detector by means of total reflection.

14. A measuring assembly according to claim 1, wherein said supporting element including said sensor elements and said sample channel is configured as a oneway element and said second and third boundary faces are used as contact surfaces for said radiation sources and said at least one detector located in a measuring assembly.

15. A measuring assembly according to claim 1, wherein said sensor elements are responsive to different analytes.