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Thermal cycler including a temperature gradient block

DC
  • US 5,779,981 A
  • Filed: 04/19/1996
  • Issued: 07/14/1998
  • Est. Priority Date: 10/20/1993
  • Status: Expired due to Term
First Claim
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1. A method for simultaneously reacting a plurality of reaction mixtures in an apparatus including a temperature gradient block and further including first and second uniform temperature blocks, said method comprising the steps of:

  • placing reaction mixtures in a plurality of reaction wells in said gradient block, said gradient block having first and second opposing end portions, said plurality of reaction mixture wells being formed in said block between said opposing end portions;

    generating a temperature gradient across said gradient block and between said opposing end portions; and

    placing the reaction mixtures in a plurality of reaction wells in said first uniform temperature block; and

    placing the reaction mixtures in a plurality of reaction wells in said second uniform temperature block; and

    generating uniform temperatures in said uniform temperature blocks.

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