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DNA sequencing, mapping, and diagnostic processes using hybridization and stable isotope labels of DNA

  • US 5,780,232 A
  • Filed: 05/28/1996
  • Issued: 07/14/1998
  • Est. Priority Date: 05/28/1996
  • Status: Expired due to Fees
First Claim
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1. A DNA sequencing, mapping, and diagnostic process using known individual isotopes, including stable isotopes and long-lived radioactive isotopes, associated with individual oligodeoxynucleotides (ODNs) or peptide nucleic acids (PNAs), said process comprising the steps of:

  • labeling nucleotide segments or PNAs with said known isotopes of an element that does not normally occur in DNAs or ODNs;

    causing said labeled, complementary, free nucleotide segments or PNAs to hybridize to fixed nucleotide segments or PNAs, said fixed nucleotide segments or PNAs being immobilized in distinct positions on a hybridization surface in an array such that said nucleotide segments or PNAs with known sequences define sequences of nucleotide segments or PNAs that become hybridized;

    rinsing non-hybridized labeled nucleotide segments or PNAs from said hybridization array; and

    using mass spectrometric methods to analyze the presence and position on said array of said hybridized, labeled complementary nucleotide segments or PNAs and identifying said isotopes.

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