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Artificial mismatch hybridization

  • US 5,780,233 A
  • Filed: 06/06/1996
  • Issued: 07/14/1998
  • Est. Priority Date: 06/06/1996
  • Status: Expired due to Term
First Claim
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1. A process for hybridizing an oligonucleotide to a first nucleic acid target, the method comprising the steps of:

  • providing an oligonucleotide having a nucleic acid sequence complementary in part to the first target, but comprising at least one artificial mismatch relative to the first target and having a nucleic acid sequence complementary in part to a second target, but comprising at least one artificial mismatch and a true mismatch relative to the second target; and

    combining the oligonucleotide and the first target under selected hybridization conditions to form a first duplex, wherein the first duplex has a melting temperature 1 to 25 C.°

    higher than that of a second duplex that would form under the same hybridization conditions between the oligonucleotide and a second nucleic acid target, where the oligonucleotide also comprises a true mismatch relative to the second target and where the true mismatch and the artificial mismatch are separated from one another by three or four nucleotide positions and where the artificial mismatch has base stacking properties of a natural nucleoside.

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