Sepsis, adult respiratory distress syndrome, and systemic inflammatory response syndrome diagnostic

• US 5,780,237 A
• Filed: 10/12/1994
• Issued: 07/14/1998
• Est. Priority Date: 10/12/1994
• Status: Expired due to Fees

• Current Assignee: Cell Therapeutics, Inc.
• Original Assignee: Cell Therapeutics, Inc.
• Inventors: Bursten, Stuart L., Federighi, David A.
• Primary Examiner(s): Hutzell, Paula K.
• Assistant Examiner(s): DUFFY, PATRICIA ANN
• Application Number:
  US08/321,483
• Time in Patent Office: 1,371 Days
• Field of Search: 435/7.32, 435/7.1, 436/71, 436/161, 436/162, 436/164, 436/178, 436/811
• US Class Current: 435/7.1
• CPC Class Codes: G01N 33/92 involving lipids, e.g. cholesterol , lipoproteins, or their receptors steroid hormones G01N33/743

Abstract

There is disclosed a diagnostic assay for adult respiratory distress syndrome (ARDS), sepsis, multiple organ dysfunction (MOD) and systemic inflammatory response syndrome (SIRS), comprising (a) measuring the amount of selected unsaturated free fatty acids (FFAs) and saturated FFAs in a body fluid, and (b) determining a ratio value comprising the sum of the unsaturated FFAs divided by the sum of the saturated FFAs. There is further disclosed a diagnostic assay for ARDS, sepsis, MOD and SIRS, comprising (a) measuring the amount of 9- or 13-hydroxyoctadecadienoic acid (HODE) and 5-hydroxy-6-trans-8,11,14-cis-eicosatetraenoic acid (5-HETE) in a body fluid, and (b) determining a ratio value of HETE and HODE.

Claims

1. A diagnostic assay for adult respiratory distress syndrome (ARDS), sepsis, multiple organ dysfunction (MOD) and systemic inflammatory response syndrome (SIRS), comprising (a) measuring an amount of unsaturated free fatty acid (FFA) selected from the group consisting of linoleate, oleate, and aracidonate and an amount of saturated free fatty acid (FFA) selected from the group consisting of myristate, palmitate, and stearate in a body fluid, and (b) determining an increased ratio value comprising the amount of selected unsaturated FFA divided by the amount of the selected saturated FFA as compared to a normal value, is diagnostic ARDS, MOD and SIRS.

2. A diagnostic assay of claim 1, wherein the unsaturated FFA is linoleate and oleate and the saturated FFA is palmitate.

3. The diagnostic assay of claim 1 wherein the body fluid is plasma, urine, sweat, saliva or tears.

4. The diagnostic assay of claim 1 wherein measuring is conducted using at least one of a technique selected from the group consisting of HPLC (high performance liquid chromatography), GC (gas chromatography), TLC (thin layer chromatography) and immunoassays using antibodies specific for a particular FFA.

5. The diagnostic assay of claim 2, wherein the body fluid is plasma.

6. The diagnostic assay of claim 3, wherein the body fluid is plasma.