Method for producing improved sensor
First Claim
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1. A method of producing an electrode membrane combination comprising the steps of:
- (1) Forming a solution containing reservoir lipids comprising within a same molecule an attachment region, hydrophobic regions, and an hydrophilic region positioned between the attachment region and the hydrophobic region, and optionally a head group attached to the hydrophobic region made from the hydrophilic region; and
spacer compounds comprising within the same molecule a hydrophilic group and an attachment group;
(2) contacting the electrode with the solution from step (1), the composition of the electrode and the attachment regions being selected such that the attachment regions chemisorbed to the electrode;
(3) rinsing the electrode;
(4) contacting the coated electrode from step (3) with a solution of lipid and ionophore in a carrier solvent containing less than 2% of an alkane; and
(5) adding an aqueous solution to the electrode from step (4).
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Abstract
The present invention relates to a novel method for producing an electrode membrane combination. This novel method employs compound including a linker lipid for use in attaching a membrane including a plurality of ionophores to an electrode and providing a space between the membrane and the electrode, the electrode being either in part or totally made up of the linker lipid. The linker lipid comprises within the same molecule a hydrophobic region capable of spanning the membrane, an attachment group used to attach the molecule to an electrode surface, and a hydrophilic region intermediate said hydrophobic region and the attachment group.
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20 Claims
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1. A method of producing an electrode membrane combination comprising the steps of:
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(1) Forming a solution containing reservoir lipids comprising within a same molecule an attachment region, hydrophobic regions, and an hydrophilic region positioned between the attachment region and the hydrophobic region, and optionally a head group attached to the hydrophobic region made from the hydrophilic region; and
spacer compounds comprising within the same molecule a hydrophilic group and an attachment group;(2) contacting the electrode with the solution from step (1), the composition of the electrode and the attachment regions being selected such that the attachment regions chemisorbed to the electrode; (3) rinsing the electrode; (4) contacting the coated electrode from step (3) with a solution of lipid and ionophore in a carrier solvent containing less than 2% of an alkane; and (5) adding an aqueous solution to the electrode from step (4). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification