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Methods for generating and screening novel metabolic pathways

  • US 5,783,431 A
  • Filed: 10/24/1996
  • Issued: 07/21/1998
  • Est. Priority Date: 04/24/1996
  • Status: Expired due to Term
First Claim
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1. A mobilizable combinatorial gene expression library, comprising a pool of expression constructs, each expression construct comprising a shuttle vector that replicates in different species or strains of host cell, said shuttle vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor organisms, and wherein the cDNA or genomic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism.

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