Forced hot air heating device

• US 5,783,439 A
• Filed: 04/30/1997
• Issued: 07/21/1998
• Est. Priority Date: 04/30/1997
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Cracauer, Raymond, Reichler, Allen
• Primary Examiner(s): Redding, David A.
• Application Number:
  US08/846,395
• Time in Patent Office: 447 Days
• Field of Search: 435/286.1, 435/286.6, 435/303.1, 435/809
• US Class Current: 435/286.1
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/1844 using fans
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 9/06 Test-tube stands; Test-tube holders
• CPC Class Codes: Y10S 435/809 Incubators or racks or holders for culture plates or containers

Abstract

A forced air heating apparatus for providing automatic cyclical heating and cooling of biological samples includes a housing and a heating chamber within the housing. The heating chamber supports a biological sample tray for supporting a plurality of sample containers. A heating device and an impeller are provided to heat the heating chamber and to direct heated air over the sample containers. The heating chamber is sealed to prevent escape of materials and includes a vent coupled to a filter to maintain ambient pressure in the heating chamber and trap particulate materials passing through the vent.

Claims

1. An apparatus for heating biological samples to a selected temperature, said apparatus comprising:

a housing having top, bottom, opposite side walls and at least one end wall, said housing having at least one air inlet for receiving outside air into said housing;

a heating chamber positioned in said housing and providing an air space between said housing and an outer surface of said heating chamber, said heating chamber having a sample opening for receiving biological samples;

a closure member for closing said sample opening in said heating chamber and hermetically sealing said heating chamber;

at least one heating element positioned on an outer surface of said heating chamber for heating an interior of said heating chamber;

a vent in said heating chamber for maintaining said heating chamber substantially at ambient pressure during a heating cycle; and

a filter coupled to said vent for capturing particulate materials escaping from said heating chamber.

2. The apparatus of claim 1, wherein said filter is a particulate air filter for capturing particles of 0.22 microns or larger.

3. The apparatus of claim 1, further comprising a cooling fan for drawing cooling air into said housing to cool said heating chamber during a cooling cycle.

4. The apparatus of claim 1, wherein said heating chamber is stainless steel.

5. The apparatus of claim 1, wherein said heating chamber comprises an integrally formed top wall, a bottom wall, two opposite side walls, and a rear wall defining an open ended cavity, wherein said open ended cavity is closed by said closure member.

6. The apparatus of claim 5, wherein said vent comprises a conduit extending from one of said walls to said filter.

7. The apparatus of claim 1, wherein said at least one heating element substantially surrounds said heating chamber.

8. The apparatus of claim 5, wherein said at least one heating element contacts said top, bottom and opposite side walls.

9. The apparatus of claim 1, further comprising at least one air impeller disposed in said heating chamber for uniformly circulating air in said heating chamber.

10. The apparatus of claim 1, wherein said impeller is driven by a motor mounted adjacent an external surface of said heating chamber.

11. The apparatus of claim 10, wherein said motor comprises a rotatable drive magnet and said impeller comprises a rotatable magnet rotatably driven by said drive magnet.

12. The apparatus of claim 11, further comprising a speed detection device coupled to said motor and a signal indicator device for indicating a first speed of said motor when said impeller is rotating and for indicating a second speed of said motor when said impeller is not being driven by said motor.

13. The apparatus of claim 9, further comprising at least one air deflector positioned in said heating chamber for dispersing circulating air in said heating chamber.

14. The apparatus of claim 1, further comprising:

a lock for locking said closure member in a closed position; and

a lock control unit, coupled to said lock, for actuating said lock.

15. The apparatus of claim 1, further comprising a rack removably received in said heating chamber, said rack having a base member and a member spaced from said base member and having a plurality of first holes for receiving sample containers, said base and top member having a plurality of second holes for uniformly circulating hot air around said sample containers.

16. The apparatus of claim 1, further comprising a temperature sensing device coupled to an outer surface of said heating chamber, for sensing a temperature of said heating chamber.

17. The apparatus of claim 1, further comprising a control unit for actuating said heating element and said drive motor for a preselected amount of time during a heating cycle to heat said biological samples at a first preselected temperature, and for deactivating said heating element and continuing actuation of said drive motor to circulate air in said heating chamber during a cooling cycle until a selected second temperature is obtained.

18. An apparatus for heating biological samples, said apparatus comprising:

a closed and sealed heating chamber having an access opening;

a closure member for closing and hermetically sealing said access opening;

a heating element on an external surface of said heating chamber for heating an internal air space of said heating chamber;

a vent in said heating chamber for maintaining substantially ambient pressure in said heating chamber when said heating chamber is heated;

a filter device coupled to said vent for filtering gases passing through said vent and collecting particulate materials from said gases;

a rotatable and magnetically driven air impeller mounted in said heating chamber for circulating air in said heating chamber;

a drive motor adjacent an external surface of said heating chamber, said drive motor having a rotatable drive magnet for driving said impeller; and

a programmable control device for actuating said heating element and drive motor for a selected time period to heat said heating chamber to a predetermined first temperature during a heating cycle and for deactivating said heating element during a cooling cycle.

19. The apparatus of claim 18, wherein said heating chamber is an integrally formed member having a top wall, a bottom wall, two opposite side walls and a rear wall, wherein said heating element surrounds said top, side and bottom walls and said vent extends through said rear wall.

20. The apparatus of claim 18, further comprising a conduit extending from said vent to said filter device, and wherein said filter device is a particulate air filter for capturing particulates of 0.22 microns or larger.

21. The apparatus of claim 18, further comprising a mounting bracket for supporting said drive motor and spacing said drive motor and drive magnet from said heating chamber.

22. The apparatus of claim 18, further comprising an air deflector in said heating chamber for deflecting air circulating in said heating chamber.

23. The apparatus of claim 18, further comprising a lock, for locking said closure member, wherein said lock is actuated by said control device for preventing opening of said closure member until a predetermined heating cycle is completed and said heating chamber cools to a second temperature.

24. The apparatus of claim 18, further comprising a temperature sensing device coupled to an external surface of said heating chamber.

25. The apparatus of claim 18, further comprising a cooling fan for passing cooling air through said housing and around said heating chamber during a cooling cycle to cool said heating chamber.

26. The apparatus of claim 18, further comprising:

a speed detection device coupled to said drive motor for detecting motor speed; and

a signal device, coupled to said speed detection device and to said control device, for producing a first signal when said motor and impeller are rotating and a second signal when said motor rotates and said impeller does not rotate.

27. An apparatus for subjecting biological samples to heating and cooling cycles, said apparatus comprising:

a housing having an air inlet for passing cooling air through said housing, said housing further including a top, bottom and opposite side walls;

a heating chamber positioned in said housing and being spaced from said top, bottom and opposite side walls for forming a first air channel substantially surrounding said heating chamber, wherein said heating chamber includes a top and bottom wall, and a sample opening for receiving said biological samples;

a closure member for closing said sample opening in said heating chamber and hermetically sealing said heating chamber;

a heating element for heating said heating chamber;

an air impeller mounted in said heating chamber for circulating heating air within said heating chamber and uniformly heating said biological samples;

a drive motor mounted outside said heating chamber and being magnetically coupled to said air impeller for driving said air impeller;

a control device for actuating said heating element and said drive motor to heat said biological sample for a preselected amount of time during a heating cycle, and for deactivating said heating element while continuing actuation of said drive motor during a cooling cycle to circulate air within said heating chamber to cool said heating chamber and biological sample; and

a sample tray for supporting a plurality of biological samples, said sample tray being removably received in said heating chamber.

28. The apparatus of claim 27, further comprising:

a vent in said heating chamber for maintaining substantially ambient pressure in said heating chamber; and

a particulate air filter coupled to said vent for capturing particulate materials of 0.22 microns or larger vented through said vent.

29. The apparatus of claim 27, further comprising an air deflector in said heating chamber for deflecting moving air from said impeller.

30. The apparatus of claim 29, wherein said air deflector is positioned adjacent said air impeller for directing air toward said bottom wall of said heating chamber.

31. The apparatus of claim 29, further comprising a lock for locking said closure member, wherein said lock is actuated by said control device for preventing opening of said closure member until a heating and cooling cycle is completed.

32. The apparatus of claim 29, further comprising a cooling fan for drawing cooling air through said housing to cool said heating chamber, said cooling fan being coupled to said control device and being actuated during a cooling cycle.

33. The apparatus of claim 29, further comprising a speed detection device coupled to said motor and a signal indicator device for indicating a first speed of said motor when said impeller is rotating and for indicating a second speed of said motor when said impeller is not being driven by said motor.