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Chemical preparation of high specific activity nucleotide probes

  • US 5,783,685 A
  • Filed: 03/21/1996
  • Issued: 07/21/1998
  • Est. Priority Date: 12/16/1988
  • Status: Expired due to Term
First Claim
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1. A chemical synthesis of a labeled nucleotide probe comprising the steps of:

  • a) providing a first target recognition moiety comprising a nucleotide sequence of at least about 15 nucleotide bases, said sequence containing at least one 5'"'"' end and 3'"'"' end,b) chemically altering said first target recognition moiety to contain a reactive functionality at said 5'"'"' end, said 3'"'"' end or both, andc) providing a second signal generating moiety, having at least one terminus containing a functionality that is capable of reacting with at least one reactive functionality provided on said first target recognition moiety, andd) chemically reacting said first target recognition moiety now containing reactive functionality with said at least one terminus of said second signal generating moiety, to chemically join these two moieties and produce said labeled nucleotide probe;

    wherein said reactive functionality of step (b) is a carboxyl group and said terminus of said signal generating moiety is a hydroxyl group.

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