Apparatus for injecting avian embryo muscle tissue in ovo
First Claim
1. An apparatus for simultaneously injecting muscle tissue of chicken embryos in a plurality of eggs during the last quarter of incubation, said device comprising:
- engaging means for engaging said plurality of eggs;
injection means cooperating with said engaging means for inserting an elongate needle through the shells of said eggs along an essentially linear path of travel to a depth of about 7/8 inch to 1.5 inch into the shoulder or breast of said embryo; and
positioning means for positioning said elongate injection needle at the large end of said egg at an angle of about 1 to 5 degrees of offset from the long axis of said egg so that said needle is directed toward the shoulder or breast of said embryo.
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0 Petitions
Accused Products
Abstract
A method has been developed for the injection of muscle tissue of an embryonic chicken in ovo. An apparatus for such injection in a plurality of eggs simultaneously has also been developed, where the apparatus is composed of a an engaging means for the eggs, an injection means to insert an elongate needle through the eggshell to a depth of about 7/8 inch to 1.5 inch into the shoulder or breast of the embryo, and a positioning means to position the needle about 1 to 5 degrees of offset of the long axis of the egg. The apparatus can delivery a variety of substances to the muscle tissue of the embryonic chicken in ovo.
88 Citations
17 Claims
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1. An apparatus for simultaneously injecting muscle tissue of chicken embryos in a plurality of eggs during the last quarter of incubation, said device comprising:
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engaging means for engaging said plurality of eggs; injection means cooperating with said engaging means for inserting an elongate needle through the shells of said eggs along an essentially linear path of travel to a depth of about 7/8 inch to 1.5 inch into the shoulder or breast of said embryo; and positioning means for positioning said elongate injection needle at the large end of said egg at an angle of about 1 to 5 degrees of offset from the long axis of said egg so that said needle is directed toward the shoulder or breast of said embryo. - View Dependent Claims (2)
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3. A method of introducing a substance into the muscle of a chicken in ovo, comprising:
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a) obtaining a chicken egg, wherein said egg contains a chicken embryo in its last quarter of incubation prior to hatch, b) positioning an elongate injection needle at the large end of the egg at an angle offset about 1 to 5 degrees from the long axis of said egg, said angle selected so that the needle is directed toward the shoulder or breast of said embryo, then c) inserting said needle through the shell of said egg along an essentially linear path of travel to a depth of about 7/8 inch to 1.5 inch into the shoulder or breast of said embryo, and then d) injecting said substance into the egg through said needle. - View Dependent Claims (4, 5, 6, 7, 8)
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9. An apparatus for simultaneously injecting muscle tissue of chicken embryos in a plurality of eggs during days 17 to 19 of incubation, said device comprising:
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engaging means for engaging said plurality of eggs; injection means cooperating with said engaging means for inserting an elongate needle through the shells of said eggs along an essentially linear path of travel to a depth of about 7/8 inch to 1.5 inch into the shoulder or breast of said embryo; and positioning means for positioning said elongate injection needle at the large end of said egg at an angle of about 1 to 5 degrees of offset from the long axis of said egg so that said needle is directed toward the shoulder or breast of said embryo. - View Dependent Claims (10)
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11. A method of introducing a substance into the muscle of a chicken in ovo, comprising:
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a) obtaining a chicken egg, wherein said egg contains a 17-19 day chicken embryo, b) positioning an elongate injection needle at the large end of the egg at an angle offset about 1 to 5 degrees from the long axis of said egg, said angle selected so that the needle is directed toward the shoulder or breast of said embryo, then c) inserting said needle through the shell of said egg along an essentially linear path of travel to a depth of about 7/8 inch to 1.5 inch into the shoulder or breast of said embryo, and then d) injecting said substance into the egg through said needle. - View Dependent Claims (12, 13, 14, 15, 16, 17)
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Specification