Apparatus for injecting avian embryo muscle tissue in ovo

• US 5,784,992 A
• Filed: 02/01/1995
• Issued: 07/28/1998
• Est. Priority Date: 01/27/1992
• Status: Expired due to Term

• Current Assignee: North Carolina State University (University of North Carolina System)
• Original Assignee: North Carolina State University (University of North Carolina System), Embrex Incorporated (Zoetis Incorporated)
• Inventors: Petitte, James, Ricks, Catherine A.
• Primary Examiner(s): Crouch, Deborah
• Application Number:
  US08/383,703
• Time in Patent Office: 1,273 Days
• Field of Search: 119/6.8, 119/174, 514/44, 514/885, 435/172.3
• US Class Current: 119/6.8
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 2227/30 Bird
• CPC Class Codes: A01K 2267/02 Animal zootechnically ameliorated
• CPC Class Codes: A01K 2267/03 Animal model, e.g. for test or diseases
• CPC Class Codes: A01K 67/0275 Genetically modified vertebrates, e.g. transgenic
• CPC Class Codes: A61K 31/70 Carbohydrates; Sugars; Derivatives thereof sorbitol A61K31/047
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C12N 15/8509 for producing genetically modified animals, e.g. transgenic

Abstract

A method has been developed for the injection of muscle tissue of an embryonic chicken in ovo. An apparatus for such injection in a plurality of eggs simultaneously has also been developed, where the apparatus is composed of a an engaging means for the eggs, an injection means to insert an elongate needle through the eggshell to a depth of about 7/8 inch to 1.5 inch into the shoulder or breast of the embryo, and a positioning means to position the needle about 1 to 5 degrees of offset of the long axis of the egg. The apparatus can delivery a variety of substances to the muscle tissue of the embryonic chicken in ovo.

Claims

1. An apparatus for simultaneously injecting muscle tissue of chicken embryos in a plurality of eggs during the last quarter of incubation, said device comprising:

engaging means for engaging said plurality of eggs;

injection means cooperating with said engaging means for inserting an elongate needle through the shells of said eggs along an essentially linear path of travel to a depth of about 7/8 inch to 1.5 inch into the shoulder or breast of said embryo; and

positioning means for positioning said elongate injection needle at the large end of said egg at an angle of about 1 to 5 degrees of offset from the long axis of said egg so that said needle is directed toward the shoulder or breast of said embryo.

2. An apparatus according to claim 1, wherein said engaging means comprises suction means for simultaneously lifting a plurality of individual eggs.

3. A method of introducing a substance into the muscle of a chicken in ovo, comprising:

a) obtaining a chicken egg, wherein said egg contains a chicken embryo in its last quarter of incubation prior to hatch,

b) positioning an elongate injection needle at the large end of the egg at an angle offset about 1 to 5 degrees from the long axis of said egg, said angle selected so that the needle is directed toward the shoulder or breast of said embryo, then

c) inserting said needle through the shell of said egg along an essentially linear path of travel to a depth of about 7/8 inch to 1.5 inch into the shoulder or breast of said embryo, and then

d) injecting said substance into the egg through said needle.

4. A method according to claim 3, said method further comprising the step of withdrawing said needle along said essentially linear path of travel;

and wherein said step of injecting said substance is carried out concurrently with said step of withdrawing said needle so that said substance is administered along said path of travel.

5. A method according to claim 4 wherein said angle is from 2 to 3 degrees.

6. A method according to claim 4, wherein said needle is inserted to a depth sufficient to pass into an through the shoulder or breast of said embryo.

7. A method according to claim 4 wherein said needle is inserted 7/8 inches into said egg.

8. A method according to claim 4, further comprising the step of incubating said egg to hatch.

9. An apparatus for simultaneously injecting muscle tissue of chicken embryos in a plurality of eggs during days 17 to 19 of incubation, said device comprising:

engaging means for engaging said plurality of eggs;

injection means cooperating with said engaging means for inserting an elongate needle through the shells of said eggs along an essentially linear path of travel to a depth of about 7/8 inch to 1.5 inch into the shoulder or breast of said embryo; and

positioning means for positioning said elongate injection needle at the large end of said egg at an angle of about 1 to 5 degrees of offset from the long axis of said egg so that said needle is directed toward the shoulder or breast of said embryo.

10. An apparatus according to claim 9, wherein said engaging means comprises suction means for simultaneously lifting a plurality of individual eggs.

11. A method of introducing a substance into the muscle of a chicken in ovo, comprising:

a) obtaining a chicken egg, wherein said egg contains a 17-19 day chicken embryo,

b) positioning an elongate injection needle at the large end of the egg at an angle offset about 1 to 5 degrees from the long axis of said egg, said angle selected so that the needle is directed toward the shoulder or breast of said embryo, then

c) inserting said needle through the shell of said egg along an essentially linear path of travel to a depth of about 7/8 inch to 1.5 inch into the shoulder or breast of said embryo, and then

d) injecting said substance into the egg through said needle.

12. A method according to claim 11, said method further comprising the step of withdrawing said needle along said essentially linear path of travel;

and wherein said step of injecting said substance is carried out concurrently with said step of withdrawing said needle so that said substance is administered along said path of travel.

13. A method according to claim 12 wherein said angle is from 2 to 3 degrees.

14. A method according to claim 12, wherein said needle is inserted to a depth sufficient to pass into and through the shoulder or breast of said embryo.

15. A method according to claim 12 wherein said needle is inserted 7/8 inches into said egg.

16. A method according to claim 12 wherein said substance comprises a DNA molecule.

17. A method according to claim 12, further comprising the step of incubating said egg to hatch.