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Systematic evolution of ligands by exponential enrichment: tissue selex

  • US 5,789,157 A
  • Filed: 05/03/1995
  • Issued: 08/04/1998
  • Est. Priority Date: 06/11/1990
  • Status: Expired due to Term
First Claim
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1. A method for identifying nucleic acid ligands to a protein component of a biological tissue comprising:

  • a) preparing a candidate mixture of nucleic acids;

    b) contacting said candidate mixture of nucleic acids with said biological tissue, wherein nucleic acids having an increased affinity to the biological tissue relative to the candidate mixture may be partitioned from the remainder of the candidate mixture and wherein said nucleic acids have a specific affinity to a protein component of said biological tissue;

    c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and

    d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with an increased affinity and an increased specificity for binding to said protein, whereby nucleic acid ligands to a protein component of a biological tissue are identified.

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