Developmental embryonic mouse cDNA libraries

• US 5,789,158 A
• Filed: 05/18/1995
• Issued: 08/04/1998
• Est. Priority Date: 06/30/1992
• Status: Expired due to Fees

• Current Assignee: The Wistar Institute
• Original Assignee: The Wistar Institute
• Inventors: Johnson, Dabney, Knowles, Barbara B., Solter, Davor, Rothstein, Jay L.
• Primary Examiner(s): Campbell, Eggerton A.
• Application Number:
  US08/439,410
• Time in Patent Office: 1,174 Days
• Field of Search: 935/56, 935/77, 935/78, 536/23.1
• US Class Current: 435/6.16
• CPC Class Codes: C12N 15/1096 cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: C12Q 1/6876 Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• CPC Class Codes: C12Q 2600/158 Expression markers

Abstract

The present invention provides a number of cDNA libraries constructed from unfertilized eggs and 2-cell, 8-cell and blastocyst stage embryos, as well as a number of novel genes expressed in the 2-cell libraries.

Claims

1. Murine embryonic cDNA library constructed from unfertilized eggs ATCC #69022.

2. Murine embryonic cDNA library constructed from 2-cell stage preimplantation embryos ATCC #69027.

3. Murine embryonic cDNA library constructed from 8-cell stage preimplantation embryos ATCC #69024.

4. Murine embryonic cDNA library constructed from blastocyst-stage preimplantation embryos ATCC #69026.

5. Murine embryonic subtraction cDNA library designated 2CLS-I ATCC #69025.

6. Murine embryonic subtraction cDNA library designated 2CLS-II ATCC #69023.

7. A method for identifying a novel gene or gene fragment comprising the steps of:

synthesizing a DNA probe comprising a DNA sequence homologous to at least one DNA sequence within a murine embryonic cDNA library selected from the group consisting of UESL ATCC #69022, BSL ATCC #69026, 2CSL ATCC #69027, and 8CSL ATCC #69024,

labeling the DNA probe, and

hybridizing the labeled DNA probe to the selected library, said label permitting identification of the gene or gene fragment.