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Optical detection of position of oligonucleotides on large DNA molecules

  • US 5,789,167 A
  • Filed: 02/13/1996
  • Issued: 08/04/1998
  • Est. Priority Date: 09/10/1993
  • Status: Expired due to Fees
First Claim
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1. A method for analyzing a sample oligonucleotide sequence comprising:

  • (a) contacting a said sample oligonucleotide sequence with an anchor sequence comprising an oligonucleotide sequence which is immobilized to a support and which hybridizes with said sample oligonucleotide sequence and with a probe comprising an oligonucleotide sequence which hybridizes to a target oligonucleotide sequence to be detected in a suitable buffer to form a complex;

    (b) subjecting said complex to a field which moves unbound oligonucleotide sequences away from said anchor sequence in the direction of said field, wherein said field is a magnetic field and a magnetic particle is attached to said sample oligonucleotide sequence or said probe or said field is an electric field; and

    (c) determining whether said probe is bound to said sample oligonucleotide sequence.

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