Recombinant yeasts for effective fermentation of glucose and xylose

• US 5,789,210 A
• Filed: 11/08/1993
• Issued: 08/04/1998
• Est. Priority Date: 11/08/1993
• Status: Expired due to Term

• Current Assignee: Purdue Research Foundation (The Trustees of Purdue University (d/b/a Purdue University))
• Original Assignee: Purdue Research Foundation (The Trustees of Purdue University (d/b/a Purdue University))
• Inventors: Ho, Nancy W. Y., Tsao, George T.
• Primary Examiner(s): Guzo, David
• Application Number:
  US08/148,581
• Time in Patent Office: 1,730 Days
• Field of Search: 435/172.3, 435/183, 435/194, 435/254.21, 435/320.1, 435/163, 435/69.1, 435/172.1, 435/254.1, 435/254.11, 435/254.2, 435/161, 435/162, 435/165, 536/23.1, 536/23.2, 536/23.7, 536/23.74
• US Class Current: 435/163
• CPC Class Codes: C12N 9/0006 acting on CH-OH groups as donors (1.1)
• CPC Class Codes: C12N 9/1205 Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

Described are recombinant yeasts containing genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase, and DNA molecules, vectors and methods useful for producing such yeasts. The recombinant yeasts effectively ferment xylose to ethanol, and preferred yeasts are capable of simultaneously fermenting glucose and xylose to ethanol thereby taking full advantage of these two sugar sources as they are found in agricultural biomass.

Claims

1. A recombinant yeast containing introduced genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase and which ferments xylose to ethanol.

2. The recombinant yeast of claim 1 wherein the yeast also ferments glucose to ethanol.

3. The recombinant yeast of claim 2 wherein the yeast is of the genus Saccharomyces.

4. The recombinant yeast of claim 3 wherein said genes are fused to non-glucose-inhibited promoters and the yeast simultaneously ferments glucose and xylose to ethanol.

14. The method of claim 2 wherein the yeast is of the genus Saccharomyces.

15. The method of claim 14 wherein said genes are fused to non-glucose-inhibited promoters and the yeast simultaneously ferments glucose and xylose to ethanol.

5. A recombinant DNA molecule comprising genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase.

6. The recombinant DNA molecule of claim 5 wherein said genes are fused to non-glucose-inhibited promoters.

7. A vector effective for transforming yeast and comprising genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase.

8. The vector of claim 7 wherein said genes are fused to non-glucose-inhibited promoters.

9. A method for obtaining a recombinant yeast which ferments xylose to ethanol, comprising introducing DNA into a yeast so as to cause the yeast to have introduced genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase.

10. The method of claim 9 wherein said introduced DNA comprises genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase.

11. The method of claim 9 wherein said yeast is of the genus Saccharomyces.

12. The method of claim 10 wherein the medium also contains glucose and the yeast also ferments said glucose to ethanol.

13. A method for fermenting xylose to ethanol, comprising fermenting a xylose-containing medium with a recombinant yeast containing introduced genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase and which ferments xylose to ethanol.

16. A method for fermenting glucose to ethanol, comprising fermenting a glucose-containing medium with a recombinant yeast containing introduced genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase and which ferments xylose and glucose to ethanol.

17. The method of claim 16 wherein said medium also contains xylose.

18. The method of claim 17 wherein said yeast is of the genus Saccharomyces.

19. A recombinant yeast containing genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase, wherein said genes are fused to non-glucose-inhibited promoters and wherein said yeast ferments xylose to ethanol.

20. The recombinant yeast of claim 19 wherein said yeast also ferments glucose to ethanol.