Methods for rapid equalibration of dissolved gas composition

• US 5,789,253 A
• Filed: 03/10/1997
• Issued: 08/04/1998
• Est. Priority Date: 07/13/1994
• Status: Expired due to Term

• Current Assignee: Abbott Point Of Care Incorporated (Abbott Laboratories)
• Original Assignee: i STAT Corporation (Abbott Laboratories)
• Inventors: Zelin, Michael P., Rogers, Joseph W., Lauks, Imants R., Pierce, Raymond J.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): CARRILLO, BIBI SHARIDAN
• Application Number:
  US08/816,006
• Time in Patent Office: 512 Days
• Field of Search: 204/403, 204/415, 204/431, 204/432, 436/8, 436/9, 436/11, 436/16, 436/63, 436/68, 436/138, 436/150, 436/151, 436/168, 436/164, 422/68.1, 422/81, 422/82.02, 422/82.03, 422/82.04, 250/577, 73/61.41, 73/1.03, 73/1.04, 73/1.05
• US Class Current: 436/68
• CPC Class Codes: G01N 33/4925 measuring blood gas content, e.g. O2, CO2 , HCO3
• CPC Class Codes: Y10T 436/10 Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
• CPC Class Codes: Y10T 436/100833 Simulative of a gaseous composition
• CPC Class Codes: Y10T 436/102499 Blood gas standard or control
• CPC Class Codes: Y10T 436/106664 Blood serum or blood plasma standard or control
• CPC Class Codes: Y10T 436/209163 Dissolved or trace oxygen or oxygen content of a sealed environment

Abstract

An apparatus and method of equilibrating the dissolved gas composition of an aqueous fluid to reflect the predetermined gas composition contained in a gas equilibration reservoir. The equilibrated aqueous fluid can be used in a method of determining the concentration of a dissolved gas in a fluid sample. In one embodiment, the method is used to control the equilibrated gas composition of a calibrant fluid which, in turn, is used to measure the concentration of a dissolved gas, such as oxygen and carbon dioxide, in a sample fluid, such as whole blood.

Claims

1. A method for equilibrating a dissolved calibrant gas in an aqueous calibrant fluid, which is adapted to be used to calibrate a gas sensor within a housing, comprising:

providing a housing containing an aqueous calibrant fluid adapted to contain at least one calibrant gas dissolved therein,

providing a gas sensor within said housing, which is adapted to measure a concentration of dissolved gas in said aqueous calibrant fluid when said sensor is in direct contact with said aqueous calibrant fluid;

providing a gas phase, comprising at least one calibrant gas, in said housing and out of direct contact with said gas sensor;

forming an interface between said aqueous calibrant fluid and said gas phase positioned out of direct contact with said gas sensor, wherein said interface is substantially proximate to said gas sensor; and

equilibrating said aqueous calibrant fluid with said gas phase comprising said at least one calibrant gas;

whereby forming an equilibrated calibrant fluid containing said at least one calibrant gas dissolved therein.

2. A method as claimed in claim 1, further comprising calibrating the gas sensor by contacting said gas sensor with the dissolved, calibrant gas in said equilibrated calibrant fluid, while maintaining said gas phase out of direct contact with said gas sensor.

3. A method as claimed in claim 1 wherein said housing comprises a conduit and said method further comprises flowing either said gas phase or said calibrating fluid through said conduit into contact with the other under conditions sufficient to form said interface.

4. A method as claimed in claim 3 which further comprises:

initially filling said conduit with said gas phase; and

then

charging said conduit with said calibrant fluid to an extent sufficient to at least cover said gas sensor;

whereby charging of said conduit with said calibrant fluid moves said gas phase out of contact with said gas sensor and forming an interface between said gas phase and said calibrant fluid proximate to, but out of contact with, said gas sensor.

5. The method as claimed in claim 1 further comprising determining the position of said interface optically.

6. The method as claimed in claim 1 further comprising determining the position of said interface by a conductivity sensor.

7. The method of claim 1 in which said gas phase comprises an ambient atmosphere.

8. The method of claim 4 further comprising determining the position of said interface optically.

9. The method of claim 4 further comprising determining the position of said interface by a conductivity sensor.

10. The method of claim 4 in which said conduit additionally comprises a conductivity sensor.

11. A method of calibrating a gas sensor, which is disposed within a housing, said method comprising;

disposing a gas sensor within said housing;

disposing a gas phase of predetermined composition in said housing;

disposing an aqueous calibrant fluid in said housing positioned so as to entirely cover said gas sensor;

forming an interface between said gas phase and said calibrant fluid;

positioning said interface proximate to but out of contact with said gas sensor;

equilibrating said gas phase with said calibrant fluid under such conditions as to form an equilibrated gas composition in at least a portion of said calibrant fluid proximate to said gas sensor; and

calibrating said gas sensor with said equilibrated gas composition of said calibrant fluid.