Blood glucose strip having reduced sensitivity to hematocrit
First Claim
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1. A reagent strip for measuring glucose concentration in a sample of whole blood that contains red cells, comprising:
- (a) an anisotropic membrane having a sample side with relatively large pores and a testing side with relatively smaller pores, the membrane being adapted to accept the sample on the sample side and pass it toward the testing side; and
(b) a testing reagent impregnated in the membrane, the testing reagent comprising(i) a component for creating hydrogen peroxide from glucose and oxygen,(ii) an indicator for reacting with the hydrogen peroxide to cause a change in the color of the indicator, and(iii) an acrylic acid polymer, of molecular weight at least about 750,000, whose concentration is in a range from about 0.1% to about 2% w/v,whereby any effect of the red blood cells on the glucose concentration measurement is reduced by the acrylic acid polymer.
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Abstract
A reagent strip for measuring glucose concentration in whole blood has reduced interference of hematocrit with the glucose measurement. When a biological fluid contacts the strip, it causes, in a reagent impregnated in the strip, a color change that is a measure of the glucose concentration in the fluid. However, the color change is also affected by the red blood cell concentration (hematocrit), thereby reducing the accuracy of the glucose measurement. The hematocrit effect is reduced by adding to the reagent an acrylic acid polymer.
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Citations
13 Claims
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1. A reagent strip for measuring glucose concentration in a sample of whole blood that contains red cells, comprising:
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(a) an anisotropic membrane having a sample side with relatively large pores and a testing side with relatively smaller pores, the membrane being adapted to accept the sample on the sample side and pass it toward the testing side; and (b) a testing reagent impregnated in the membrane, the testing reagent comprising (i) a component for creating hydrogen peroxide from glucose and oxygen, (ii) an indicator for reacting with the hydrogen peroxide to cause a change in the color of the indicator, and (iii) an acrylic acid polymer, of molecular weight at least about 750,000, whose concentration is in a range from about 0.1% to about 2% w/v, whereby any effect of the red blood cells on the glucose concentration measurement is reduced by the acrylic acid polymer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for measuring glucose concentration in a sample of whole blood that contains red blood cells, which comprises the steps of:
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(a) providing an anisotropic membrane, which (i) has a sample side having relatively large pores and a testing side having relatively smaller pores and (ii) is impregnated with a testing reagent that can react with glucose to cause a change in color of the testing side, the reagent comprising glucose oxidase, a peroxidase, an oxidizable dye or dye couple, and an acrylic acid polymer, of molecular weight at least about 750,000, whose concentration is in a range from about 0.1% to about 2% w/v; (b) applying the blood to the sample side of the membrane; and (c) measuring the change in color of the testing side to determine the glucose concentration in the blood. - View Dependent Claims (12, 13)
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Specification