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High level expression of proteins

  • US 5,795,737 A
  • Filed: 09/22/1995
  • Issued: 08/18/1998
  • Est. Priority Date: 09/19/1994
  • Status: Expired due to Term
First Claim
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1. A synthetic gene encoding a green fluorescent protein, wherein at least one non-preferred or less preferred codon in the natural gene encoding said protein has been replaced by a preferred codon encoding the same amino acid, said preferred codons being selected from the group consisting of gcc, cgc, aac, gac, tgc, cag, ggc, cac, atc, ctg, aag, ccc, ttc, agc, acc, tac, and gtg, said less preferred codons being selected from the group consisting of ggg, att, ctc, tcc, and gtc, said non-preferred codons being all codons other than said preferred codons and said less preferred codons, wherein said synthetic gene permits the expression of said green fluorescent protein in a mammalian host cell at a level which is at least 110% of that expressed by said natural gene in an in vitro mammalian cell culture system under identical conditions.

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