Carboxylic acid reductase, and methods of using same
First Claim
1. A substantially purified, biologically-derived carboxylic acid reductase, isolated from Nocardia sp. strain NRRL 5646 having the N-terminal amino acid sequence of SEQ ID NO:
- 1, wherein the enzyme is characterized by its ability to biocatalytically reduce a carboxylic acid, or a derivative thereof, to its corresponding product(s) and intermediary by-product(s), and further characterized by its ability to reduce vanillic acid to vanillin.
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Abstract
The present invention is directed to a novel, biologically-derived carboxylic acid reductase, also referred to herein as an aryl-aldehyde oxidoreductase, that has been isolated and purified from Nocardia sp. strain NRRL 5646, and to methods of using the carboxylic acid reductase as a biocatalyst for the reduction of carboxylic acids or their derivatives to the corresponding useful product(s). In a preferred embodiment, the invention is directed to biochemically-produced vanillin, and to the methods of its production by the biocatalytic reduction of vanillic acid, or a precursor or derivative thereof, in a reaction comprising the substantially purified, biologically-derived carboxylic acid reductase.
25 Citations
5 Claims
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1. A substantially purified, biologically-derived carboxylic acid reductase, isolated from Nocardia sp. strain NRRL 5646 having the N-terminal amino acid sequence of SEQ ID NO:
- 1, wherein the enzyme is characterized by its ability to biocatalytically reduce a carboxylic acid, or a derivative thereof, to its corresponding product(s) and intermediary by-product(s), and further characterized by its ability to reduce vanillic acid to vanillin.
- View Dependent Claims (2, 3, 4, 5)
Specification
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- Resources
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Current AssigneeThe University of Iowa Research Foundation
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Original AssigneeUniversity of Iowa Research Foundation
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InventorsLi, Tao, Rosazza, John P. N.
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Primary Examiner(s)Wax, Robert A.
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Assistant Examiner(s)Stole, Einar
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Application NumberUS08/813,437Time in Patent Office526 DaysField of Search435/147, 435/189, 426/44US Class Current435/189CPC Class CodesC12N 9/0008 acting on the aldehyde or o...
