Gene encoding endoglycoceramidase

• US 5,795,765 A
• Filed: 06/28/1996
• Issued: 08/18/1998
• Est. Priority Date: 06/29/1995
• Status: Expired due to Term

• Current Assignee: Takara Shuzo Company Limited (Ningbo Junsheng Investment Group Co., Ltd.)
• Original Assignee: Takara Shuzo Company Limited (Ningbo Junsheng Investment Group Co., Ltd.)
• Inventors: Izumi, Yoshiya, Kurome, Yoko, Ito, Makoto, Kato, Ikunoshin, Sano, Mutsumi, Izu, Hiroyuki
• Primary Examiner(s): Wax, Robert A.
• Assistant Examiner(s): Stole, Einar
• Application Number:
  US08/672,571
• Time in Patent Office: 781 Days
• Field of Search: 435/200, 435/320.1, 435/240.2, 435/252.3, 536/23.2
• US Class Current: 435/200
• CPC Class Codes: C12N 9/2402 hydrolysing O- and S- glycosyl compounds (3.2.1)
• CPC Class Codes: C12Y 302/01123 Endoglycosylceramidase (3.2.1.123)

Abstract

An isolated DNA having a sequence encoding a polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof, and a method for producing a polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof by gene recombinant technology.

Claims

1. An isolated DNA having a sequence encoding a polypeptide possessing endoglycoceramidase activity wherein the isolated DNA comprises a DNA sequence selected from the group consisting of:

(a) a DNA sequence encoding an amino acid sequence of SEQ ID NO;

1 or Seg ID NO;

3;

(b) a DNA sequence of SEQ ID NO;

2 or SEQ ID NO;

4; and

(c) a DNA sequence which hybridizes to (a) or (b) above.

2. The isolated DNA according to claim 1, wherein the polypeptide is derived from a strain of the genus Rhodococcus.

3. The isolated DNA according to claim 2, wherein the polypeptide is derived from Rhodococcus sp. M-777.

4. A recombinant DNA which comprises the isolated DNA of claim 1.

5. A vector which comprises the recombinant DNA of claim 4.

6. The vector according to claim 5, wherein the recombinant DNA is operably linked to a promoter.

7. A cell of a procaryote or eucaryote transformed with the vector of claim 5.

8. A method for producing a polypeptide possessing endoglycoceramidase activity, comprising the steps of:

(a) culturing the cell of claim 7; and

(b) recovering the polypeptide possessing endoglycoceramidase activity from the culture obtained in Step (a).

9. A synthetic oligonucleotide probe or primer which specifically hybridizes with the isolated DNA of claim 1.