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Compositions and methods for cell transformation

  • US 5,795,870 A
  • Filed: 11/07/1994
  • Issued: 08/18/1998
  • Est. Priority Date: 12/13/1991
  • Status: Expired due to Fees
First Claim
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1. A method of enhancing the transformability of a host cell comprising contacting a host cell with a compound of the formula (I):

  • ##STR2## in which R1 can be an H, OH, OR5, NH2, NHR6 or NR6 R7 ;

    R2 and R3 may be the same or different and can be an H, OH or OR5 ;

    R4 can be CONH2, CONHR6, CONR6 R7, CH2 NH2, CH2 NHR6, CH2 NR6 R7, CO2 --Y--NH2, CO2 --Y--NHR6, or CO2 --Y--NR6 R7 ;

    R5 is a protected or unprotected glycosyl moiety comprising 1-10 monosaccharide units in which the glycosidic linkage at the anomeric carbon atom of each monosaccharide unit is independently alpha or beta;

    NH2, NHR6, and NR6 R7 represent an unsubstituted amino group, a monosubstituted amino group, and a disubstituted amino group, respectively, in which R6 and R7 may be the same or different and represent a hydrocarbon group comprising 1-15 carbon atoms substituted with one or more unsubstituted, monosubstituted or disubstituted amino groups;

    Y represents a linear or branched alkylene group comprising 1-10 carbon atoms;

    n is an integer from 0-10;

    or its acid addition or quaternary ammonium salt;

    provided that said compound comprises at least one R6 group as defined above; and

    (b) allowing said compound to remain in contact with said host cell while exposing said host cell to transforming nucleic acid.

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