Method of optically measuring component in solution

• US 5,796,476 A
• Filed: 06/26/1996
• Issued: 08/18/1998
• Est. Priority Date: 06/28/1995
• Status: Expired due to Fees

• Current Assignee: Kyoto Daiichi Kagaku Company Limited
• Original Assignee: Kyoto Daiichi Kagaku Company Limited
• Inventors: Dou, Xiaoming, Wang, Yung Xiang
• Primary Examiner(s): Evans, F. L.
• Assistant Examiner(s): Smith, Zandra V.
• Application Number:
  US08/672,026
• Time in Patent Office: 783 Days
• Field of Search: 356/317, 356/318, 356/301, 250/461.2, 250/339.01-339.15
• US Class Current: 356/301
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/65 Raman scattering

Abstract

A sample solution containing protein is irradiated with excitation light of a single wavelength which is emitted from a light source, so that light scattered from the sample solution is received and separated into its spectral components in a spectroscope, thereby obtaining light scattering spectra. Protein is quantitatively measured through intensity of a light scattering spectrum in a shift wavenumber of 100 to 3100 cm^-1 with respect to the excitation wavelength among the light scattering spectra or an integral value in a proper range therein. As to a body fluid sample, the sample is irradiated with excitation light and Raman scattering spectral intensity values are measured at a plurality of wavenumbers in an arbitrary wavenumber range, and a plurality of components in the sample are analyzed simultaneously by multivariate regression analysis.

Claims

1. A measuring method comprising the steps of:

for a plurality of components of a body fluid to be measured, previously selecting a wavenumber having an excellent correlation between the concentration of a component and light scattering spectral intensity as a measuring wavenumber which is specific to said component;

irradiating a body fluid sample with excitation light for measuring light scattering spectral intensity at each said measuring wavenumber as to said each component to be measured or light scattering spectral cumulative intensity in each measuring wavenumber range; and

simultaneously qualitatively/quantitatively analyzing respective said components in said body fluid through a calibration curve being previously prepared as to said light scattering spectral intensity at each said measuring wavenumber or said light scattering spectral cumulative intensity in said each measuring wavenumber range and the concentration of said component.

2. The measuring method in accordance with claim 1, wherein

a plurality of components in said body fluid sample are simultaneously qualitatively/quantitatively analyzed by multivariate regression analysis.

3. In the measuring method in accordance with claim 1, wherein

said wavenumber having an excellent correlation between the concentration of said each component and said light scattering spectral intensity has a correlation coefficient R of at least 0.8,

said correlation coefficient R being calculated as follows:

##EQU4## where xi represents the concentration of each point of said component in said body fluid, yi represents Raman scattering spectral intensity with respect to xi, X represents an average value of the concentration of each point of said component in said body fluid, and Y represents an average value of said Raman scattering spectral intensity.

4. The measuring method in accordance with claim 3, wherein the correlation coefficient R is at least 0.9.

5. In the measuring method in accordance with claim 1, wherein

at least one of albumin, globulin, hemoglobin, glucose, lithium acetoacetate, β

-hydroxybutyric acid, acetone, ditaurobilirubin, urobilin, sodium nitrite, urea, uric acid, folic acid, ascorbic acid, vitamin B₂, creatine monohydrate, creatinine, α

-amylase, β

-amylase and ammonia is contained as a body fluid component to be measured, measuring wavenumbers or measuring wavenumber ranges for respective said components being

selected from around 590 to 650 cm^-1, around 800 to 860 cm^-1, and around 2342 to 2402 cm^-1 for albumin,

selected from around 158 to 218 cm^-1, around 402 to 462 cm^-1, around 623 to 683 cm^-1, around 807 to 867 cm^-1, around 822 to 862 cm^-1, around 1353 to 1413 cm¹, around 1562 to 1622 cm^-1, around 1847 to 1907 cm^-1, around 2095 to 2155 cm^-1, around 2340 to 2400 cm^-1 and around 2403 to 2463 cm^-1 for globulin,

selected from around 807 to 867 cm^-1, around 893 to 953 cm^-1, around 1353 to 1413 cm^-1, around 1562 to 1622 cm^-1, around 1840 to 1900 cm^-1, around 2095 to 2155 cm^-1, around 2340 to 2400 cm^-1, and around 2403 to 2463 cm^-1 for hemoglobin,

selected from around 200 to 554 cm^-1, around 810 to 944 cm^-1, and around 2590 to 2940 cm^-1 for glucose,

selected from around 799 to 859 cm^-1, around 1353 to 1413 cm^-1, around 1840 to 1900 cm^-1, around 2347 to 2407 cm^-1, and around 2907 to 2967 cm^-1 for lithium acetoacetate,

selected from around 1420 to 1480 cm^-1, around 1557 to 1617 cm^-1, around 1600 to 1660 cm^-1, and around 2870 to 2971 cm^-1 for β

-hydroxybutyric acid,

selected from around 775 to 845 cm^-1, around 1050 to 1110 cm^-1, around 1207 to 1267 cm^-1, around 1399 to 1459 cm^-1, 1680 to 1740 cm^-1, and around 2911 to 2971 cm^-1 for acetone,

selected from around 927 to 987 cm^-1, around 1233 to 1293 cm^-1, and around 1586 to 1616 cm^-1 for ditaurobilirubin,

selected as an arbitrary position from 332 to 2900 cm^-1 for urobilin,

selected from around 783 to 843 cm^-1, and around 1318 to 1378 cm^-1 for sodium nitrite,

selected from around 501 to 561 cm^-1, around 567 to 627 cm^-1, around 978 to 1048 cm^-1, around 1033 to 1093 cm^-1, around 1138 to 1198 cm^-1, and around 1583 to 1643 cm^-1 for urea,

selected from around 640 to 700 cm^-1 for uric acid,

selected from around 631 to 691 cm^-1, around 816 to 876 cm^-1, around 901 to 961 cm^-1, around 1355 to 1415 cm^-1, around 1562 to 1622 cm^-1, around 1847 to 1907 cm^-1, around 2093 to 2153 cm^-1, around 2339 to 2399 cm^-1, and around 400 to 2460 cm^-1 for folic acid,

selected from around 800 to 860 cm^-1, around 1673 to 1733 cm^-1, and around 2919 to 2979 cm^-1 for ascorbic acid,

selected from around 404 to 464 cm^-1, around 626 to 686 cm^-1, around 798 to 858 cm^-1, around 860 to 920 cm^-1, around 1317 to 1377 cm^-1, around 1546 to 1606 cm^-1, around 1834 to 1894 cm^-1, around 2106 to 2166 cm^-1, around 2360 to 2420 cm^-1, and around 2419 to 2479 cm^-1 for vitamin B₂,

selected from around 800 to 860 cm^-1, and around 1563 to 1623 cm^-1 for creatine monohydrate,

selected from around 538 to 598 cm^-1, 580 to 640 cm^-1, around 665 to 725 cm^-1, around 817 to 877 cm^-1, around 868 to 928 cm^-1, around 1021 to 1081 cm^-1, around 1550 to 1610 cm^-1, around 2868 to 2900 cm^-1, around 2900 to 2950 cm^-1, and around 2950 to 2996 cm^-1 for creatinine,

selected from around 393 to 453 cm^-1, around 631 to 691 cm^-1, around 792 to 852 cm^-1, around 868 to 928 cm^-1, around 1333 to 1393 cm^-1, around 1546 to 1606 cm^-1, around 1834 to 1894 cm^-1, and around 2334 to 2394 cm^-1 for β

-amylase,

selected from around 376 to 436 cm^-1, around 622 to 682 cm^-1, around 783 to 843 cm^-1, around 868 to 928 cm^-1, around 1334 to 1394 cm^-1, around 1546 to 1606 cm^-1, around 1834 to 1894 cm^-1, and around 2665 to 2725 cm^-1 for β

-amylase,

selected from around 3190 to 3250 cm^-1, around 3292 to 3350 cm^-1, and 3377 to 3437 cm^-1 for ammonia,

selected from around 404 to 464 cm^-1, around 805 to 865 cm^-1, around 1014 to 1074 cm^-1, around 1438 to 1498 cm^-1, and around 2966 to 3026 cm^-1 for inositol,

selected from around 466 to 526 cm^-1, 835 to 895 cm^-1, around 1032 to 1092 cm^-1, 1237 to 1297 cm^-1, around 1332 to 1392 cm^-1, around 1438 to 1498 cm^-1, and around 2946 to 3006 cm^-1 for galactose, and

selected from around 569 to 629 cm^-1, around 772 to 832 cm^-1, around 1044 to 1106 cm^-1, around 1237 to 1297 cm^-1, around 1438 to 1498 cm^-1, and around 2966 to 2996 cm^-1 for fructose.

6. A method of measuring albumin by irradiating a sample solution containing albumin with excitation light of a single wavelength, receiving scattered light from said sample solution and separating the same into its spectral components for obtaining light scattering spectra, and quantitatively measuring albumin through peak intensity of a spectrum being shifted from said excitation wavelength to 810 to 840 cm^-1, or an integral value of a proper range of a spectrum being shifted from an excitation wavelength of 632.8 nm to 256 to 1620 cm^-1 or from an excitation wavelength of 514.5 nm to 837 to 3060 cm^-1.

7. A method of measuring γ

-globulin by irradiating a sample solution containing γ

-globulin with excitation light of a single wavelength, receiving scattered light from said sample solution and separating the same into its spectral components for obtaining light scattering spectra, and quantitatively measuring γ

-globulin through peak intensity of a spectrum being shifted from said excitation wavelength to 175 to 195 cm^-1, 425 to 450 cm^-1, 640 to 670 cm^-1, 820 to 845 cm^-1, 845 to 870 cm^-1, 1370 to 1400 cm^-1 1575 to 1620 cm^-1, 1850 to 1900 cm^-1, 2000 to 2200 cm^-1, 2350 to 2400 cm^-1 or 2400 to 2460 cm^-1.

8. A method of measuring hemoglobin by irradiating a sample solution containing hemoglobin with excitation light of a single wavelength, receiving scattered light from said sample solution and separating the same into its spectral components for obtaining light scattering spectra, and quantitatively measuring hemoglobin peak intensity of a spectrum being shifted from said excitation wavelength to 640 to 670 cm^-1, 820 to 845 cm^-1, 1370 to 1400 cm^-1, 1575 to 1620 cm^-1, 1850 to 1900 cm^-1, 2000 to 2200 cm^-1, 2350 to 2400 cm^-1 or 2400 to 2460 cm^-1.