Electrochemical biosensor
First Claim
1. An electrochemical sensor for the detection of an analyte in a fluid test sample which comprises from the bottom up:
- a) an insulating base plate;
b) an electrode layer on said base plate;
c) a lid of a deformable material which provides a concave area in the central portion thereof in such a manner that, when mated with the base plate, the lid and base plate form a capillary space in which the electrode layer is available for contact with the fluid test sample which is drawn into the capillary space by capillary action.
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Accused Products
Abstract
The present invention concerns an electrochemical sensor made up of an insulating base having an electrode layer on its surface and a lid of deformable material which comprises a concave area in the central portion thereof, so that when it is mated with the base, the lid and base form a capillary space containing the electrode layer. When the electrode layer is in operative contact with a reaction layer comprising an enzyme which will cause the production of mobile electrons when contacted with a suitable analyte, the concentration of analyte, e.g. glucose in blood, can be measured by measuring the current created by the flow of mobile electrons when contacted with a suitable analyte, the concentration of analyte, e.g. glucose in blood, can be measured by measuring the current created by the flow of mobile electrons.
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Citations
25 Claims
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1. An electrochemical sensor for the detection of an analyte in a fluid test sample which comprises from the bottom up:
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a) an insulating base plate; b) an electrode layer on said base plate; c) a lid of a deformable material which provides a concave area in the central portion thereof in such a manner that, when mated with the base plate, the lid and base plate form a capillary space in which the electrode layer is available for contact with the fluid test sample which is drawn into the capillary space by capillary action. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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- 14. A method for the preparation of an electrochemical sensor which comprises mating a base which carries an electrode structure on its surface with a lid of a deformable material which provides a concave space to form three sides of a capillary space which lid and base provide an electrochemical sensor having a capillary space which is open to the atmosphere with an electrode structure on the surface of the base and exposed to the capillary space.
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22. A method for the production of a multiplicity of electrochemical sensors which comprises:
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a) providing a sheet of a deformable material and a base sheet of an insulating material bearing a pattern of formed electrodes on its surface, wherein the sheet of deformable material has a substantially uniform layer of a thermally fusible material on a surface thereof; b) embossing the sheet of deformable material to form a series of concave zones in the sheet which mirror the pattern of electrodes on the base sheet; c) mating the sheet of deformable material to the base sheet so that the concave zones in the sheet of deformable material substantially encompass the electrodes and sealing these two sheets together by the application of sufficient heat and pressure to fuse the fusible adhesive; and d) punching individual sections from the fused sheets using a die of an appropriate shape to provide individual sensors comprising a capillary space circumscribed on three sides by the concave zone from the sheet of deformable material and on the bottom by the punched out portion of the base sheet to provide a sensor having a capillary space open to the atmosphere within which resides an electrode which is available for contact with a fluid test sample which is drawn into the capillary space by capillary action. - View Dependent Claims (23, 24, 25)
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Specification