Apparatus for improved luminescence assays using particle concentration and chemiluminescence detection
First Claim
1. An apparatus for measurement of an analyte of interest based upon measurement of chemiluminescence comprising:
- (a) a cell consisting essentially of a sample volume having a vertical columnar zones, inlet means and outlet means for generating a magnetic field positioned proximate to a single collection zone, said collection zone being below a substantial volume of said cell and said columnar zone, and said field being sufficient to collect said analyte of interest at said collection zone when any such analyte of interest is bound directly or indirectly to a magnetic particle; and
(b) means to measure the chemiluminescence generated at the collection zone.
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Accused Products
Abstract
What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and/or the assay-performance-substance; incubating the composition to form a complex which includes a particle and the labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.
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Citations
22 Claims
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1. An apparatus for measurement of an analyte of interest based upon measurement of chemiluminescence comprising:
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(a) a cell consisting essentially of a sample volume having a vertical columnar zones, inlet means and outlet means for generating a magnetic field positioned proximate to a single collection zone, said collection zone being below a substantial volume of said cell and said columnar zone, and said field being sufficient to collect said analyte of interest at said collection zone when any such analyte of interest is bound directly or indirectly to a magnetic particle; and (b) means to measure the chemiluminescence generated at the collection zone. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. An assay apparatus for measurement of an analyte of interest based upon measurement of chemiluminescence comprising:
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(a) a cell consisting essentially of a sample volume having inlet means and outlet means and a single collection zone; (b) means for generating a magnetic field at said collection zone, wherein said means for generating said magnetic field is positioned proximate to said cell and said field is sufficient to collect said analyte at said collection zone when any such analyte of interest is bound directly or indirectly to a magnetic particle; and (c) means to measure the chemiluminescence generated at the collection zone. - View Dependent Claims (22)
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Specification