Recombinant lactobacillus for fermentation of xylose to lactic acid and lactate

• US 5,798,237 A
• Filed: 10/10/1995
• Issued: 08/25/1998
• Est. Priority Date: 10/10/1995
• Status: Expired due to Term

• Current Assignee: Alliance For Sustainable Energy LLC
• Original Assignee: Midwest Research Institute
• Inventors: Franden, Mary Ann, Mc Millan, James D., Zhang, Min, Picataggio, Stephen K., Finkelstein, Mark
• Primary Examiner(s): Elliott, George C.
• Assistant Examiner(s): Wang, Andrew
• Application Number:
  US08/541,632
• Time in Patent Office: 1,050 Days
• Field of Search: 435/252.3, 435/252.9, 435/320.1, 435/139, 435/243, 435/248
• US Class Current: 435/139
• CPC Class Codes: C12N 15/746 for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
• CPC Class Codes: C12N 9/1205 Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
• CPC Class Codes: C12N 9/90 Isomerases (5.)
• CPC Class Codes: C12P 7/56 Lactic acid

Abstract

A recombinant Lactobacillus MONT4 is provided which has been genetically engineered with xylose isomerase and xylulokinase genes from Lactobacillus pentosus to impart to the Lactobacillus MONT4 the ability to ferment lignocellulosic biomass containing xylose to lactic acid.

Claims

1. A homofermentative microorganism Lactobacillus MONT4 containing exogenous genes encoding xylose isomerase and xylukokinase which functions with transaldolase and transketolase enzymes in the pentose phosphate pathway for homofermentating xylose to lactic acid, wherein the microorganism homofermentates xylose to lactic acid in high yields of 94% of maximum yield based upon an amount of consumed sugar and wherein said microorganism without said exogenous genes being incapable of homofermentating said xylose to lactic acid.

2. The microorganism of claim 1, containing exogenous genes encoding xylose isomerase and xylulokinase.

3. The microorganism of claim 2, wherein said genes encoding xylose isomerase and xylulokinase are obtained from Lactobacillus pentosus.

4. The microorganism of claim 1, wherein said genes are located on a vector.

5. The microorganism of claim 4, wherein said vector is a plasmid.

8. A process of producing lactic acid or lactate in high yields from lignocellulosic biomass by adding the microorganism of claim 1 into said lignocellulosic biomass to ferment sugars to lactic acid or lactate.

9. The process according to claim 8 wherein the lignocellulosic biomass contains xylose.

10. The process according to claim 8 wherein the lignocellulosic biomass contains xylose and glucose.

11. The process according to claim 8 wherein the lignocellulosic biomass contains xylose, glucose and arabinose.

12. The microorganism of claim 11, wherein the transaldolase and transketolase are inducible in the presence of arabinose.

6. A vector containing genes encoding xylose isomerase and xylulokinase obtained form Lactobacillus pentosus, but not containing the xylR gene.

7. The vector of claim 6, wherein the vector is a plasmid.