Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid

• US 5,798,259 A
• Filed: 11/20/1996
• Issued: 08/25/1998
• Est. Priority Date: 05/15/1992
• Status: Expired due to Fees

• Current Assignee: Sagami Chemical Research Center
• Original Assignee: Sagami Chemical Research Center
• Inventors: Yamada, Akiko, Yazawa, Kazunaga, Kondo, Kiyosi, Kato, Seishi
• Primary Examiner(s): Prouty, Rebecca E.
• Application Number:
  US08/752,929
• Time in Patent Office: 643 Days
• Field of Search: 536/23.2, 536/23.1, 435/183, 435/136, 435/134, 435/252.3, 435/325, 435/320.1
• US Class Current: 435/252.3
• CPC Class Codes: C12N 9/93 Ligases (6)
• CPC Class Codes: C12P 7/6432 Eicosapentaenoic acids [EPA]

Abstract

There is provided an advantageous process for production of EPA by a gene recombinant technique wherein genes coding for biosynthesis enzymes for eicosapentaenoic acid (EPA) useful as pharmaceuticals, agrochemicals, foods, feeds or the like is obtained from microorganisms.

EPA is produced by obtaining genes coding for eicosapentaenoic acid (EPA) biosynthesis enzymes, constructing a plasmid by joining the genes to a vector, transforming E. coli with the plasmid, and culturing the transformed E. coli.

Claims

1. An isolated DNA comprising nucleotides 5661 to 34631 in SEQ ID NO:

1, coding for a group of eicosapentaenoic acid synthesis enzymes.

11. A plasmid comprising an isolated DNA according to claim 1.

13. A host cell transformed with a plasmid according to claim 11.

2. An isolated DNA comprising nucleotides 8081 to 9441, 12314 to 13084, and 13889 to 32520 in SEQ ID NO:

1, coding for a group of eicosapentaenoic acid synthesis enzymes.

3. An isolated DNA comprising nucleotides 8081 to 9441, 12314 to 13084, 13889 to 32520, and 34627 to 35559 in SEQ ID NO:

1, coding for a group of eicosapentaenoic acid synthesis enzymes.

4. An isolated DNA comprising nucleotides 8081 to 9441, 12314 to 13084, and 13889 to 35559 in SEQ ID NO:

1, coding for a group of eicosapentaenoic acid synthesis enzymes.

5. An isolated DNA comprising nucleotides 8081 to 9441, 9681 to 13084, and 13889 to 32520 in SEQ ID NO:

1, coding for a group of eicosapentaenoic acid synthesis enzymes.

6. An isolated DNA comprising nucleotides 8081 to 9441, 9681 to 13084, and 13889 to 32520, and 34627 to 35564, coding for a group of eicosapentaenoic acid synthesis enzymes.

7. An isolated DNA comprising nucleotides 8081 to 9441, 9681 to 13084, and 13889 to 35564, coding for a group of eicosapentaenoic acid synthesis enzymes.

8. An isolated DNA coding for a group of eicosapentaenoic acid synthesis enzymes comprising the amino acid sequences shown in SEQ ID NOs:

5, 11, 13, 15 and 17.

9. An isolated DNA comprising the nucleotide sequences shown in SEQ ID NOs:

4, 10, 12, 14 and 16, coding for a group of eicosapentaenoic acid synthesis enzymes.

10. An isolated DNA comprising a nucleotide sequence coding for an enzyme having an amino acid sequence shown in SEQ ID NO:

13 or 15, for elongating the chain of stearic acid to arachidic acid.

12. A plasmid comprising an isolated DNA according to claim 10.

14. A host cell transformed with a plasmid according to claim 12.