Method for chromosomes classification

• US 5,798,262 A
• Filed: 04/18/1997
• Issued: 08/25/1998
• Est. Priority Date: 02/22/1991
• Status: Expired due to Term

• Current Assignee: Applied Spectral Imaging Ltd.
• Original Assignee: Applied Spectral Imaging Ltd.
• Inventors: Katzir, Nir, Wine, David, Cabib, Dario, Garini, Yuval
• Primary Examiner(s): Ketter, James
• Assistant Examiner(s): Brusca, John S.
• Application Number:
  US08/844,516
• Time in Patent Office: 494 Days
• Field of Search: 435/6, 435/287.2, 395/131, 359/368
• US Class Current: 435/287.2
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: G01J 2003/2866 Markers; Calibrating of scan
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/12 Generating the spectrum; Monochromators
• CPC Class Codes: G01J 3/1256 using acousto-optic tunable filter; acousto-optic elements or systems G02F1/11, G02F1/33
• CPC Class Codes: G01J 3/26 using multiple reflection, e.g. Fabry-Perot interferometer, variable interference filters
• CPC Class Codes: G01J 3/2823 Imaging spectrometer
• CPC Class Codes: G01J 3/4406 Fluorescence spectrometry
• CPC Class Codes: G01J 3/453 by correlation of the amplitudes
• CPC Class Codes: G01N 2021/6423 Spectral mapping, video display
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025
• CPC Class Codes: G01N 33/56966 Animal cells
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: G06V 10/88 Image or video recognition using optical means, e.g. reference filters, holographic masks, frequency domain filters or spatial domain filters
• CPC Class Codes: G06V 20/69 Microscopic objects, e.g. biological cells or cellular parts
• CPC Class Codes: G06V 20/695 Preprocessing, e.g. image segmentation

Abstract

A method for finding L internal reference vectors for classification of L chromosomes or portions of chromosomes of a cell, the L chromosomes or portions of chromosomes being painted with K different fluorophores or combinations thereof, wherein K basic chromosomes or portions of chromosomes of the L chromosomes or portions of chromosomes are each painted with only one of the K different fluorophores, whereas the other L-K of the L chromosomes or portions of chromosomes are each painted with a different combination of the K different fluorophores, the method comprising the steps of (a) using a multi-band collection device for measuring a first vector for each pixel of each of the L chromosomes or portions of chromosomes; (b) identifying pixels belonging to each of the K basic chromosomes or portions of chromosomes and defining the pixels as basic pixels, so as to obtain K basic classes of basic pixels; (c) using at least one basic pixel from each of the K basic classes for obtaining K basic vectors, the K basic vectors being K internal reference vectors; (d) using the K basic vectors for identifying pixels belonging to the other L-K chromosomes or portions of chromosomes; and (e) using the pixels belonging to the other L-K chromosomes or portions of chromosomes for calculating the other L-K internal reference vectors, thereby finding all of the L internal reference vectors. A method for classification of L chromosomes or portions of chromosomes of a cell similarly painted using the above method for finding L internal reference vectors, and using the L reference vectors for classification of each of the pixels into one of L classification classes. And, images presenting color chromosomes.

Claims

1. An apparatus for displaying chromosomes, the apparatus comprising:

(a) a multi-band collection device being supplemented with algorithms for providing a visual presentation device with an image of all chromosomes or portions of chromosomes of a cell, each of said chromosomes or portions of chromosomes being painted with a different fluorophore or a combination of fluorophores, said image presenting said chromosomes or portions of chromosomes in different distinctive colors, wherein each of said chromosomes or portions of chromosomes is attributed one of said different distinctive colors; and

(b) a visual presentation device for displaying said chromosomes or portions of chromosomes.

2. An apparatus for displaying chromosomes, the apparatus comprising:

(a) a multi-band collection device being supplemented with algorithms for providing a visual presentation device with a composite image of at least some chromosomes or portions of chromosomes of a cell, each of said chromosomes or portions of chromosomes being painted with a different fluorophore or a combination of fluorophores and banded with a chromosome banding technique to obtain a characteristic banding pattern for each of said chromosomes or portions of chromosomes, said image presenting said chromosomes or portions of chromosomes in distinctive colors, wherein each of said chromosomes or portions of chromosomes is attributed one of said distinctive colors and further wherein each of said distinctive colors is acquired an intensity pattern in accordance with said banding pattern of each of said chromosomes or portion of chromosomes, so that both said distinctive colors and said banding patterns of each of said chromosomes or portions of chromosomes are overlaid; and

(b) a visual presentation device for displaying said chromosomes or portions of chromosomes.

3. An apparatus for displaying chromosomes, the apparatus comprising:

a multi-band collection device being supplemented with algorithms for providing a visual presentation device with an image of at least some chromosomes or portions of chromosomes of a cell, each of said chromosomes or portions of chromosomes being painted with a different fluorophore or a combination of fluorophores and banded with a chromosome banding technique to obtain a characteristic banding pattern and shape for each of said chromosomes or portions of chromosomes, said image presenting said chromosomes or portions of chromosomes in distinctive colors, wherein each of said chromosomes or portions of chromosomes is attributed one of said distinctive colors and further wherein each of said chromosomes or portion of chromosomes is acquired said characteristic shape, so that both said distinctive colors and said shapes of each of said chromosomes or portions of chromosomes are overlaid; and

(b) a visual presentation device for displaying said chromosomes or portions of chromosomes.

4. An apparatus for displaying chromosomes, the apparatus comprising:

(a) a multi-band collection device being supplemented with algorithms for providing a visual presentation device with a composite image of a chromosome or portion of chromosome, said chromosome or portion of chromosome being presented in a color, said color including a banding pattern of alternating lighter and darker bands, both said color and said pattern of bands being indicative of said chromosome portion of chromosome identification; and

(b) a visual presentation device for displaying said chromosomes or portions of chromosomes.

5. An apparatus for displaying chromosomes, the apparatus comprising:

(a) a multi-band collection device being supplemented with algorithms for providing a visual presentation device with an image of a chromosome or portion of chromosome, said chromosome or portion of chromosome being presented in a color, said color being indicative to said chromosome or portion of chromosome identification, wherein said chromosome or portion of chromosome being shaped similar to its shape when branded using a chromosome banding technique; and

(b) a visual presentation device for displaying said chromosomes or portions of chromosomes.

6. An apparatus for displaying chromosomes, the apparatus comprising:

(a) a multi-band collection device being supplemented with algorithms for providing a visual presentation device with a composite image of a chromosome or portion of chromosome, said composite image including a color image and a banded image of said chromosome or portion of chromosome; and

(b) a visual presentation device for displaying said chromosomes or portions of chromosomes.