Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces

• US 5,800,690 A
• Filed: 07/03/1996
• Issued: 09/01/1998
• Est. Priority Date: 07/03/1996
• Status: Expired due to Term

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Holdings Incorporated
• Inventors: Parce, J. Wallace, Chow, Calvin Y. H.
• Primary Examiner(s): Gorgos, Kathryn L.
• Assistant Examiner(s): Noguerola, Alex
• Application Number:
  US08/678,436
• Time in Patent Office: 790 Days
• Field of Search: 204/451, 204/454, 204/457, 204/601, 204/602, 204/607, 204/608
• US Class Current: 204/451
• CPC Class Codes: G01N 27/44713 Particularly adapted electric power supply
• CPC Class Codes: G01N 27/44752 Controlling the zeta potential, e.g. by wall coatings
• CPC Class Codes: G01N 27/44773 Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: Y10S 366/02 Micromixers: segmented laminar flow with boundary mixing orthogonal to the direction of fluid propagation with or without geometry influences from the pathway
• CPC Class Codes: Y10T 29/49002 Electrical device making

Abstract

In a microfluidic system using electrokinetic forces, the present invention uses electrical current or electrical parameters, other than voltage, to control the movement of fluids through the channels of the system. Time-multiplexed power supplies also provide further control over fluid movement by varying the voltage on an electrode connected to a fluid reservoir of the microfluidic system, by varying the duty cycle during which the voltage is applied to the electrode, or by a combination of both. A time-multiplexed power supply can also be connected to more than one electrode for a savings in cost.

Claims

1. A method of using a microfluidic system having a plurality of interconnected capillary channels and a plurality of electrodes at different nodes of said capillary channels for creating electric fields in said capillary channels to electrokinetically move materials in a fluid through said capillary channels, the method comprising:

applying voltages simultaneously to at least three of said electrodes with respect to other electrodes in the system said voltages being responsive to a current at at least two of said at least three electrodes to move materials into and through one or more intersections of said plurality of channels of said system.

2. The method of claim 1 wherein said microfluidic system has more than three electrodes.

3. The method of claim 2 wherein said voltage applying step comprises controlling said voltages so that said current is substantially constant.

4. The method of claim 1, wherein the materials to be moved are charged, the charged materials moving to and from the channels of the system by electrophoresis.

5. The method of claim 1, wherein the materials to be moved are in a fluid, and the applying step results in electroosmotic movement of the fluid containing the materials to and from the channels of the system.

6. The method of claim 1, wherein the plurality of interconnected capillary channels includes at least one channel intersection, and the voltages applied at the at least three electrodes move material into the intersection from channels connected to the at least three electrodes.

7. The method of claim 1, wherein the applying step further comprises controlling the voltages applied to the at least three electrodes, such that a current between one of the at least three electrodes and another of the at least three electrodes is maintained substantially constant.

8. A method of using a microfluidic system having a plurality of capillary channels and a plurality of electrodes at different nodes of said capillary channels for creating electric fields in said capillary channels to electrokinetically move materials in a fluid through said capillary channels, the method comprising

simultaneously controlling in time an application of an electrical parameter between at least three electrodes in the system to move materials therebetween wherein the electrical parameter is current or power.

9. The method of claim 8 wherein said application is controlled such that said materials move equivalently to a constant application of said electrical parameters between said electrodes in the system.

10. The method of claim 9 wherein said application is controlled by varying a percentage of time said electrical parameter is applied.