Sealant for sample holder

• US 5,800,778 A
• Filed: 05/31/1995
• Issued: 09/01/1998
• Est. Priority Date: 05/31/1995
• Status: Expired due to Term

• Current Assignee: Biomerieux Incorporated (Compagnie Merieux Alliance SAS)
• Original Assignee: Biomerieux Incorporated (Compagnie Merieux Alliance SAS)
• Inventors: Chen, Patrick K., O'Bear, Raymond E.
• Primary Examiner(s): ALEXANDER, LYLE
• Application Number:
  US08/455,404
• Time in Patent Office: 1,189 Days
• Field of Search: 422/56, 422/58, 422/61, 422/48, 422/102, 422/104, 422/99
• US Class Current: 422/48
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/545 for laboratory containers
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: Y10T 436/108331 Preservative, buffer, anticoagulant or diluent

Abstract

An improved sealant for a biocard or other sample holder is provided. The sealant achieves low weight, good tensile strength, and high oxygen permeability while avoiding excessive fluid evaporation, speeding incubation times.

Claims

1. An apparatus for use in biological sample testing comprising:

a test sample card having a card body, said card body defining a well for receiving a fluid sample containing a microbiological agent and a reagent for said fluid sample;

a high oxygen transmissible and permeable membrane applied to said card body in a manner to cover said well and provide a high oxygen transmissible and permeable barrier between said microbiological agent and reagent in said well and an atmosphere external of said test sample card, said membrane being substantially impermeable to liquids and making an engagement to said test sample card, said membrane comprising polymethylpentene;

said high oxygen transmissible and permeable membrane permanently secured to said card body and covering said well in a manner so as to provide a closed reaction chamber for said microbiological agent and said reagent within said well,

whereby said high oxygen transmissible and permeable membrane promotes the reaction between said reagent and said microbiological agent.

2. The apparatus according to claim 1, further comprising:

an adhesive backing for fixing the high oxygen transmissible and permeable membrane to said card body of the test sample card.

3. The apparatus according to claim 1, wherein the high oxygen transmissible and permeable membrane at a thickness of 2 mils has an oxygen permeability of 4800-5000 (cc/100 in² /24 hours/25°

C.).

4. The apparatus of claim 1, wherein said reagent comprises a growth media for said microbiological agent.

5. The apparatus of claim 1, wherein said reagent comprises an antibiotic for said microbiological agent.

6. The apparatus according to claim 1, wherein the card body of said test sample card has a front surface and a rear surface, and said well defining an aperture through said card body between said front and rear surfaces.

7. The apparatus according to claim 6, wherein the test sample card comprises a plurality of sample wells arranged in an array, said high oxygen transmissible and permeable sealing membrane covering said array of sample wells.

8. A sample holder, comprising:

a sample holder body having at least one well and defining front and rear surfaces, and

a high oxygen transmissible and permeable membrane covering said well so as to provide a high oxygen transmissible and permeable barrier between a microbiological agent and a reagent loaded in said well and an atmosphere external of said sample holder, said membrane being substantially impermeable to liquids and applied to said at least one of said front and rear surfaces, said membrane comprising polymethylpentene;

said high oxygen transmissible and permeable membrane permanently secured to said sample holder body and covering said well in a manner so as to provide a closed reaction chamber for said microbiological agent and said reagent within said well.

9. The sample holder of claim 8, wherein said high oxygen transmissible and permeable sealing membrane is applied to both said front and rear surfaces of said sample holder.

10. The sample holder of claim 8, wherein said high oxygen transmissible and permeable sealing membrane has a thickness of 2 mils.

11. A biological test sample card, comprising a card body defining a plurality of sample wells, and having front and rear surfaces, said wells comprising an aperture extending between said front and rear surfaces, a high oxygen transmissible and permeable membrane applied to a portion of said front and rear surfaces so as to cover said sample wells, wherein said membrane is substantially impermeable to liquids and wherein said membrane comprises polymethylpentene.

12. The biological test sample card of claim 11, wherein said wells are arranged in an array and are loaded with reactants, said polymethylpentene membrane increasing the transmissibility of oxygen into said wells.

13. The biological test sample card of claim 12, wherein said polymethylpentene membrane is applied to said front surface and to said rear surface of said card.

14. The biological test sample card of claim 13, wherein said polymethylpentene membrane has a thickness of 2 mils.

15. An improved biological test sample card for use in optical analysis of a biological fluid sample containing a microbiological agent, comprising:

a test sample card body having front and rear surfaces and a plurality of sample wells forming apertures therethrough arranged in an array, said plurality of sample wells receiving a reagent for said microbiological agent;

a first oxygen permeable membrane applied to said front surface of said card body and covering all of said apertures in said card body, said first membrane comprising polymethylpentene, said first membrane being substantially impermeable to liquids and increasing the transmissibility of oxygen into said plurality of sample wells; and

a second membrane to said rear surface of said card body and covering all of said apertures in said card body;

said high oxygen transmissible and permeable membrane and said second membrane permanently secured to said card body and covering all of said apertures in said card body in a manner so as to provide a plurality of closed reaction chambers for said microbiological agent and said reagent.

16. The biological test sample card of claim 15, wherein said first membrane at a thickness of 2 mils have an oxygen permeability of 4800-5000 (cc/100 in² /24 hours/25°

C.).

17. The biological test sample card of claim 15, wherein said first and second membranes are adhesively attached to said front and rear surfaces of said test sample card body.