Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
First Claim
1. A set of at least 2 different fluorescent labels, said set comprising:
- a first energy transfer fluorescent label comprising at least one donor fluorophore and at least one acceptor fluorophore in energy transfer relationship separated by a distance x; and
a second energy transfer fluorescent label comprising said at least one donor fluorophore and said at least one acceptor fluorophore in energy transfer relationship separated by a distance y;
wherein;
(a) said donor fluorophores of said first and second labels are the same;
(b) said acceptor fluorophores of said first and second labels are the same; and
(c) x and y are sufficiently different so that said first and second fluorescent labels provide distinguishable fluorescent signals wherein all of the labels of said set are acceptor fluorophores are selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluoresein and cyanine dyes.
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Accused Products
Abstract
Sets of fluorescent energy transfer labels and methods for their use in multi component analysis, particularly nucleic acid enzymatic sequencing, are provided. In the subject sets, at least two of the labels are energy transfer labels comprising a common donor and acceptor fluorophore in energy transfer relationship separated by different distances and capable of providing distinguishable fluorescence emission patterns upon excitation at a common wavelength. The subject labels find particular use in a variety of multi-component analysis applications, such as probes in FISH and multi array analyses, as well as primers in nucleic acid enzymatic sequencing applications.
132 Citations
20 Claims
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1. A set of at least 2 different fluorescent labels, said set comprising:
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a first energy transfer fluorescent label comprising at least one donor fluorophore and at least one acceptor fluorophore in energy transfer relationship separated by a distance x; and a second energy transfer fluorescent label comprising said at least one donor fluorophore and said at least one acceptor fluorophore in energy transfer relationship separated by a distance y; wherein;
(a) said donor fluorophores of said first and second labels are the same;
(b) said acceptor fluorophores of said first and second labels are the same; and
(c) x and y are sufficiently different so that said first and second fluorescent labels provide distinguishable fluorescent signals wherein all of the labels of said set are acceptor fluorophores are selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluoresein and cyanine dyes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A set of four different fluorescently labeled energy transfer oligonucleotide primers, said set comprising:
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a first energy transfer fluorescently labeled oligonucleotide primer comprising a donor fluorophore and an acceptor fluorophore in energy transfer relationship separated by a distance x; a second energy transfer fluorescently labeled oligonucleotide primer comprising said donor fluorophore and said acceptor fluorophore in energy transfer relationship separated by a distance y; a third energy transfer fluorescently labeled oligonucleotide primer comprising said donor fluorophore and said acceptor fluorophore in energy transfer relationship separated by a distance z; and a fourth energy transfer fluorescently labeled oligonucleotide primer wherein said donor and acceptor fluorophores have the same fluorescence emission; wherein;
(a) said donor fluorophore of said first, second and third labels are the same;
(b) said acceptor fluorophores of said first, second and third labels are the same and selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluorescein and cyanine dyes; and
(c) x, y and z are sufficiently different so that said first through fourth fluorescently labeled oligonucleotide primers provide distinguishable fluorescence emission patterns. - View Dependent Claims (11, 12, 13)
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14. A set of four different energy transfer fluorescently labeled oligonucleotide primers for use in enzymatic sequencing, said set comprising:
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a first energy transfer fluorescently labeled oligonucleotide comprising a donor fluorophore bonded to the 5'"'"' terminus and an acceptor fluorophore bonded to the 7th nucleotide 3'"'"' from the 5'"'"' terminus; a second energy transfer fluorescently labeled oligonucleotide comprising said donor fluorophore bonded to the 5'"'"' terminus and said acceptor fluorophore bonded to the 14th nucleotide 3'"'"' from the 5'"'"' terminus; a third energy transfer fluorescently labeled oligonucleotide comprising said donor fluorophore bonded to the 5'"'"' terminus and said acceptor fluorophore bonded to the 17th nucleotide 3'"'"' from the 5'"'"' terminus; and a fourth energy transfer fluorescently labeled oligonucleotide comprising said donor fluorophore bonded to the 5'"'"' terminus and said donor fluorophore bonded to the 17th nucleotide 3'"'"' from the 5'"'"' terminus; wherein (a) said donor fluorophore of said first, second, third and fourth labeled oligonucleotides are the same; and
(b) said acceptor fluorophores of said first, second, third and fourth labeled oligonucleotides are the same and are selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluorescein and cyanine dyes. - View Dependent Claims (15, 16)
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17. A kit for use in DNA sequencing, said kit comprising:
a set of four fluorescently labeled oligonucleotide primers, wherein at least two of said oligonucleotide primers have a common donor and acceptor fluorophore in energy transfer relationship and are separated by different distances wherein all of the labels of said set are acceptor fluorophores are selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluoresein and cyanine dyes. - View Dependent Claims (18, 19, 20)
Specification