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Sets of labeled energy transfer fluorescent primers and their use in multi component analysis

  • US 5,804,386 A
  • Filed: 01/15/1997
  • Issued: 09/08/1998
  • Est. Priority Date: 01/15/1997
  • Status: Expired due to Fees
First Claim
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1. A set of at least 2 different fluorescent labels, said set comprising:

  • a first energy transfer fluorescent label comprising at least one donor fluorophore and at least one acceptor fluorophore in energy transfer relationship separated by a distance x; and

    a second energy transfer fluorescent label comprising said at least one donor fluorophore and said at least one acceptor fluorophore in energy transfer relationship separated by a distance y;

    wherein;

    (a) said donor fluorophores of said first and second labels are the same;

    (b) said acceptor fluorophores of said first and second labels are the same; and

    (c) x and y are sufficiently different so that said first and second fluorescent labels provide distinguishable fluorescent signals wherein all of the labels of said set are acceptor fluorophores are selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluoresein and cyanine dyes.

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