Synthetic receptors, libraries and uses thereof

• US 5,804,563 A
• Filed: 04/08/1996
• Issued: 09/08/1998
• Est. Priority Date: 01/13/1994
• Status: Expired due to Term

• Current Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Original Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Inventors: Li, Ge, Still, W. Clark
• Primary Examiner(s): Hutzell, Paula K.
• Assistant Examiner(s): BAKALYAR, HEATHER
• Application Number:
  US08/628,972
• Time in Patent Office: 883 Days
• Field of Search: 435/6, 435/7.1, 436/501, 436/518, 436/528, 436/529, 436/531, 436/534, 530/333, 530/334, 530/300, 536/1.11, 536/4.1, 536/23.1, 536/25.3, 536/25.32, 536/5, 514/26, 514/44, 514/48, 552/101, 552/200, 552/208, 564/1
• US Class Current: 514/26
• CPC Class Codes: B01J 2219/0054 Means for coding or tagging the apparatus or the reagents
• CPC Class Codes: B01J 2219/00572 Chemical means
• CPC Class Codes: B01J 2219/00592 Split-and-pool, mix-and-divide processes
• CPC Class Codes: C07J 41/0055 the 17-beta position being substituted by an uninterrupted chain of at least three carbon atoms which may or may not be branched, e.g. cholane or cholestane derivatives, optionally cyclised, e.g. 17-beta-phenyl or 17-beta-furyl derivatives
• CPC Class Codes: C07J 41/0061 one of the carbon atoms being part of an amide group
• CPC Class Codes: C07J 9/005 containing a carboxylic function directly attached or attached by a chain containing only carbon atoms to the cyclopenta[a]hydrophenanthrene skeleton
• CPC Class Codes: C07K 1/047 Simultaneous synthesis of different peptide species; Peptide libraries
• CPC Class Codes: C07K 5/123 Tripeptides
• CPC Class Codes: C12Q 1/6811 Selection methods for production or design of target specific oligonucleotides or binding molecules
• CPC Class Codes: C40B 70/00 Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

Abstract

The invention is directed to synthetic receptor(s) which comprises a polyfunctional organic template covalently linked to two or more oligomers which may independently be the same or different and may independently be straight chain or branched. The template may be linked to an identifier which uniquely defines the synthetic receptor. The identifier is a stable chemical molecule or a plurality of stable chemical molecules distinguishable and detectable to picomolar levels or may be an oligonucleotide. In an preferred embodiment, the template is covalently linked to a solid support which is linked to an identifier.

Claims

1. A synthetic receptor which comprises a polyfunctional organic template covalently linked to two or more oligomer which may independently be straight chained or branched oligoamide, oligoester, oligourea, oligourethane, oligoamine, oligoether, oligosulfonamide, oligophosphonamide, oligophoephonate, oligophqsphate, oligonucleotide, oligosaccharide, peptide oligomer, or a mixture of monomers thereof;

wherein each oligomer independently contains 2 to 10 monomers; and

wherein the polyfunctional organic template is a polycarbocyclic.

2. The synthetic receptor of claim 1, wherein at least one of the oligomers is a combination of two or more distinct classes of oligomers selected from the group consisting of an oligoamide, an oligoester, an oligourea, an oligourethane, an oligoamine, an oligoether, an oligosulfonamide, an oligophosphonamide, an oligophosphonate, an oligophosphate, an oligonucleotide, an oligosaccharide and a peptide oligomer.

3. The synthetic receptor of claim 1, wherein the oligomers are different.

4. The synthetic receptor of claim 1, wherein the oligomers are the same.

8. The synthetic receptor of claim 1, wherein the polyfunctional organic template is further linked to a dye, a fluorescent label or a radioactive label.

9. The synthetic receptor of claim 1, wherein the polyfunctional organic template is further linked to an identifier which uniquely defines the synthetic receptor.

10. The synthetic receptor of claim 9, wherein the identifier uniquely defines the synthesis and molecular structure of the oligomers of the synthetic receptor.

11. The synthetic receptor of claim 9, wherein the identifier is a stable chemical molecule or a plurality of stable chemical molecules distinguishable and detectable to picomolar levels.

12. The synthetic receptor of claim 9, wherein the identifier is an oligonucleotide.

5. A synthetic receptor which comprises a polyfunctional steroid template covalently linked to two or more peptide oligomers which may independently be the same or different and which may independently be straight chain or branched.

6. The synthetic receptor of claim 5, wherein the polyfunctional steroid template is ursodeoxycholic acid, hyodeoxycholic acid, alpha-apocholic acid,

7. The synthetic receptor of claim 5, wherein at least two of said two or more peptide okugomers comprise at least two amino acids.

13. The synthetic receptor of claim 5, wherein the polyfunctional steroid template is covalently linked to a solid support.

14. The synthetic receptor of claim 13, wherein the solid support is polystyrene or PEG-polystyrene particles.