Solid medium and method for DNA storage

• US 5,807,527 A
• Filed: 09/21/1995
• Issued: 09/15/1998
• Est. Priority Date: 05/29/1991
• Status: Expired due to Term

• Current Assignee: Whatman PLC (GE Aerospace)
• Original Assignee: Flinders Technologies Pty., Ltd.
• Inventors: Burgoyne, Leigh Alexander
• Primary Examiner(s): Jones, W. Gary
• Assistant Examiner(s): REES, DIANE
• Application Number:
  US08/531,597
• Time in Patent Office: 1,090 Days
• Field of Search: 435/6, 435/5, 435/91.2, 435/7.1-7.9, 536/24.3-24.33, 424/488, 424/443, 424/464
• US Class Current: 424/488
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence

Abstract

A solid medium for storage of DNA, including blood DNA, comprising a solid matrix having a compound or composition which protects against degradation of DNA incorporated into or absorbed on the matrix. Methods for storage of DNA using this solid medium, and for recovery of DNA or in situ use of DNA are also disclosed.

Claims

1. A solid medium for storage of at least one sample of DNA, the solid medium comprising:

(a) a solid matrix;

(b) an effective amount of a composition which protects against degradation of DNA adsorbed or incorporated onto said solid matrix, said composition comprising;

(i) a protein denaturing agent; and

(ii) a free radical trap.

2. The solid medium according to claim 1 wherein the composition is adsorbed or incorporated onto the solid matrix before addition of a sample of DNA.

3. The solid medium according to claim 1 wherein the free radical trap is uric acid or a urate salt.

4. The solid medium according to claim 1 wherein the protein denaturing agent is a detergent.

5. The solid medium according to claim 1 wherein the free radical trap acts as a DNA releasing agent to enhance release of DNA from said solid medium.

6. The solid medium according to claim 1 wherein the solid medium inactivates pathogenic organisms.

7. The solid medium according to claim 1 wherein the composition includes a divalent ion binding agent.

8. A solid medium for storage and release of a sample of DNA said, solid medium comprising:

a solid matrix having adsorbed or incorporated thereto

an effective amount of a protein denaturing agent and a free radical trap.

9. The solid medium according to claim 8 wherein the protein denaturing agent is a detergent.

10. The solid medium according to claim 8 wherein the free radical trap facilitates release of DNA from said solid medium.

11. The solid medium according to claim 8 wherein the free radical trap is uric acid or a urate salt.

12. The solid medium according to claim 8 wherein the protein denaturing agent is an anionic detergent.

13. The solid medium according to claim 8 wherein the DNA stored and released from the solid medium is subsequently analyzed.

14. A solid medium for storage and release of a sample of DNA applied to the solid medium, such that after the sample of DNA is applied to the solid medium, the solid medium comprises:

a sample of DNA;

a solid matrix;

a protein denaturing agent;

a divalent ion binding agent; and

a free radical trap.

15. The solid medium according to claim 14 wherein the protein denaturing agent is a detergent.

16. A composition which when adsorbed to or incorporated into a cellulose based solid matrix provides a dry solid medium for storage of a sample of DNA, the composition comprising a weak base, a chelating agent, a surfactant and optionally uric acid or a urate salt.

17. A method for storage of a sample of DNA, the method comprising:

(a) applying a sample of DNA to a dry solid medium, the dry solid medium comprising a cellulose based matrix and a composition consisting essentially of a weak base, a chelating agent, an anionic surfactant and optionally uric acid or a urate salt, wherein the composition is adsorbed on or incorporated into the solid matrix; and

(b) storing the sample of DNA applied to the dry solid medium.