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Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides

  • US 5,808,045 A
  • Filed: 06/07/1995
  • Issued: 09/15/1998
  • Est. Priority Date: 09/02/1994
  • Status: Expired due to Term
First Claim
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1. A mononucleoside 5'"'"'-triphosphate having a removable blocking moiety protecting the 3'"'"' position which is an ester and which has the following formula:

  • ##STR18## wherein R2 is a triphosphate and R is selected from the group consisting of;

    formate, benzoylformate, chloroacetate, dichloroacetate, trichloroacetate, trifluoroacetate, methoxyacetate, triphenylmethoxyacetate, phenoxyacetate, p-chlorophenoxyacetate, 2,6-dichloro-4-methylphenoxyacetate, 2,6-dichloro-4-(1,1,3,3-tetramethylbutyl)phenoxyacetate, 2,4-bis(1,1-dimethylpropyl)phenoxyacetate, chlorodiphenylacetate, p-P-phenylacetate, 3-phenylpropionate, 3-benzoylpropionate, isobutyrate, 4-oxopentanoate, pivaloate, adamanioate, crotonate, 4-methoxycrotonate, (E)-2-methyl-2-butenoate, o-(dibromomethyl)benzoate, o-(methoxycarbonyl)benzoate, p-phenylbenzoate, 2,4,6-trimethylbenzoate and α

    -naphthanoate.

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