Radiolabeled nucleotide formulations stored in an unfrozen state

• US 5,811,072 A
• Filed: 05/06/1997
• Issued: 09/22/1998
• Est. Priority Date: 04/30/1992
• Status: Expired due to Term

• Current Assignee: GE Healthcare UK Limited (Danaher Corporation)
• Original Assignee: Amersham Pharmacia Biotech UK Ltd. (Danaher Corporation)
• Inventors: Stone, Timothy, May, Christopher Charles, Buckley, Elizabeth Margaret, Price, Roger Malcolm
• Primary Examiner(s): Hollinden, Gary E.
• Assistant Examiner(s): JONES, DAMERON LEVEST
• Application Number:
  US08/852,050
• Time in Patent Office: 504 Days
• Field of Search: 424/1.11, 424/1.65, 424/1.73, 424/1.81, 436/1, 436/8, 436/18, 436/56, 436/57, 436/504, 436/545, 436/804, 252/625, 252/644, 252/645, 252/646
• US Class Current: 424/1.73
• CPC Class Codes: A61K 2123/00 Preparations for testing in vivo
• CPC Class Codes: A61K 51/0406 Amines, polyamines, e.g. spermine, spermidine, amino acids, (bis)guanidines
• CPC Class Codes: C07B 59/00 Introduction of isotopes of elements into organic compounds ; Labelled organic compounds per se
• CPC Class Codes: C07B 59/005 Sugars; Derivatives thereof; Nucleosides; Nucleotides; Nucleic acids
• CPC Class Codes: C07B 63/04 Use of additives anti-oxidant compositions or compositions inhibiting chemical change in general C09K15/00
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: G01N 33/60 involving radioactive labelled substances
• CPC Class Codes: G01N 33/6806 Determination of free amino acids
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10T 436/13 Tracers or tags

Abstract

Additives are proposed for compositions comprising radiolabelled organic compounds e.g. 32P-labelled nucleotides. Stabilisers are selected from tryptophan, para-aminobenzoate, indoleacetate and the azole group. Dyes are selected from Sulphorhodamine B, Xylene Cyanol, Azocarmine B and New Coccine. Preferred compositions contain both stabiliser and dye.

Claims

1. A product consisting of a stabilized solution of a nucleotide labeled with a β

-emitting nuclide, said solution also containing a dye, and said solution contained in an unfrozen state within a closed vessel, which vessel is suitable for storage and shipment of the solution in an unfrozen state.

2. The product as claimed in claim 1, wherein the nucleotide is a deoxynucleoside triphosphate or ribonucleoside triphosphate.

3. The product according to claim 1, wherein the β

-emitting nuclide is ³² P, ³³ P or ³⁵ S.

4. The product according to claim 1, wherein said solution consists essentially of the nucleotide labeled with a β

-emitting nuclide, a stabilizer and the dye optionally together with a buffer.

5. The product according to claim 4, wherein the concentration of stabilizer is 10 to 100 mM.

6. The product according to claim 1, wherien the dye concentration in said solution is 50 to 400 μ

g/ml.

7. The product according to claim 1, wherein said solution is suitable for use in enzymatic reaction systems.