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Blocked compartments in a PCR reaction vessel

  • US 5,811,296 A
  • Filed: 12/20/1996
  • Issued: 09/22/1998
  • Est. Priority Date: 12/20/1996
  • Status: Expired due to Term
First Claim
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1. In a flexible reaction cuvette comprising opposed plastic sheets sealed together to define expandable compartments comprising a sample reaction chamber, a detection chamber, a waste collection chamber, a first passageway between said reaction chamber and said detection chamber, and a second passageway between said detection chamber and said collection chamber;

  • the improvement wherein said sheets are blocked together in the regions of at least said reaction chamber, said collection chamber, and said passageways by an amount sufficient to require at least 0.8 g/cm of width lineal peel-apart force when peeled at a 90 degree angle, to separate said sheets at said chambers or passageways, but insufficient to permanently adhere the sheets together by an amount that prevents liquid from entering said chambers or passageways;

    so that air bubbles are eliminated from the chambers and the passageways prior to initiating flow of liquid into them.

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