Dicationic perylene dye for precipitation and quantitation of DNA
First Claim
1. A process for the precipitation, recovery and quantitation of DNA from solutions containing DNA which comprises the steps of:
- a. adding N,N'"'"'-bis- 3,4,9,10-perylenetetracarboxylic diimide to a solution containing DNA and allowing the resulting mixture to equilibrate;
b. collecting the resulting precipitate;
c. removing weakly bound excess N,N'"'"'-bis-3,4,9,10-perylenetetracarboxylic diimide from said precipitate;
d. extracting N,N'"'"'-bis-3,4,9,10-perylenetetracarboxylic diimide from said precipitate by the addition of a solvent for N,N'"'"'-bis-3,4,9,10-perylenetetracarboxylic diimide; and
e. determining the amount of DNA precipitated by measuring either the absorbance or fluorescence of N,N'"'"'-bis-3,4,9,10-perylenetetracarboxylic diimide in the solvent extract from step d.
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Abstract
The novel dicationic dye N,N'"'"'-bis 3,3'"'"'-(dimethylamino)propylamine!-3,4,9,10-perylenetetracarboxylic diimide (DAPER) is prepared by reacting 3,4,9,10-perylenetetracarboxylic dianhydride with N,N-dimethylaminopropylamine. The dye DAPER is particularly useful in a rapid, sensitive method for the precipitation, recovery and quantitation of DNA from solutions containing DNA, which method involves the steps of (a) adding the dye DAPER to a solution containing DNA and allowing the resulting mixture to equilibrate; (b) collecting the resulting precipitate; (c) removing weakly bound DAPER from the precipitate; (d) extracting DAPER therefrom by the addition of a solvent therefor; and (e) determining the amount of DNA precipitated by measuring either the absorbance or fluorescence of DAPER in the solvent extract from step (d).
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Citations
3 Claims
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1. A process for the precipitation, recovery and quantitation of DNA from solutions containing DNA which comprises the steps of:
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a. adding N,N'"'"'-bis- 3,4,9,10-perylenetetracarboxylic diimide to a solution containing DNA and allowing the resulting mixture to equilibrate; b. collecting the resulting precipitate; c. removing weakly bound excess N,N'"'"'-bis-3,4,9,10-perylenetetracarboxylic diimide from said precipitate; d. extracting N,N'"'"'-bis-3,4,9,10-perylenetetracarboxylic diimide from said precipitate by the addition of a solvent for N,N'"'"'-bis-3,4,9,10-perylenetetracarboxylic diimide; and e. determining the amount of DNA precipitated by measuring either the absorbance or fluorescence of N,N'"'"'-bis-3,4,9,10-perylenetetracarboxylic diimide in the solvent extract from step d. - View Dependent Claims (2, 3)
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Specification