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Optical measurement apparatus and method therefor

  • US 5,811,312 A
  • Filed: 08/30/1995
  • Issued: 09/22/1998
  • Est. Priority Date: 08/31/1993
  • Status: Expired due to Fees
First Claim
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1. An optical measurement apparatus comprising:

  • a reaction vessel for holding liquids;

    an optical waveguide for propagating light therewithin, the optical waveguide forming at least one surface of the reaction vessel wherein a substance is fixed on the surface for binding to a substance to be measured;

    means for radiating an exciting light to the optical waveguide at a first predetermined angle relative to the optical waveguide;

    a fluorescent substance which produces fluorescent light when excited by the exciting light contained within the reaction vessel for labeling at least a portion of a substance to be measured which is bound to the substance fixed on the surface of the waveguide;

    a light absorbing material contained within the reaction vessel, the light absorbing material absorbing light at a wavelength of the exciting light or at a wavelength of the fluorescent light; and

    means for measuring fluorescent light which is emitted from the fluorescent substance labeling a portion of a substance to be measured which is bound to the substance fixed on the surface of the waveguide at a second predetermined angle relative to the optical waveguide.

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