Selective recognition of solutes in chromatographic media by artificially created affinity
First Claim
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1. A method for preparing an affinity chromatography medium with selective affinity for a preselected species, said method comprising:
- (a) forming a insoluble polymer matrix from a polymerizable substance in the presence of said preselected species, said polymerizable substance being non-ionizable and one with respect to which said preselected species is inert; and
(b) extracting said preselected species from said insoluble polymer matrix.
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Abstract
Chromatographic media that demonstrate an artificially created recognition of any preselected molecular species with only an affinity-type adsorption are formed by polymerization from solutions of non-ionizable monomers with a quantity of the species contained in the solution, followed by removal of the species by conventional washing. The media are of particular utility and interest when used to achieve affinity binding of proteins. Uses of the media include chromatography and the general isolation and removal of the adsorbed species.
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12 Claims
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1. A method for preparing an affinity chromatography medium with selective affinity for a preselected species, said method comprising:
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(a) forming a insoluble polymer matrix from a polymerizable substance in the presence of said preselected species, said polymerizable substance being non-ionizable and one with respect to which said preselected species is inert; and (b) extracting said preselected species from said insoluble polymer matrix. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification